文献类型：期刊文献

英文题名：A new colorimetric and fluorescent probe with a large stokes shift for rapid and specific detection of biothiols and its application in living cells

作者：Zhang, Meng-Zhao[1,2];Han, Hai-Hao[1,2];Zhang, Shao-Ze[3];Wang, Cheng-Yun[1,2];Lu, Yun-Xiang[3];Zhu, Wei-Hong[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Chem & Mol Engn, Dept Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：5

期号：44

起止页码：8780

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20174704425899);WOS:【SCI-EXPANDED(收录号:WOS:000415354000015)】；

基金：The authors are grateful for financial support from the Natural Science Foundation of Shanghai (No. 16ZR1408000), the National Key Program of China (No. 2016YFA0200302) and the Fundamental Research Funds for the Central Universities. And the authors would like to thank Dr Xiao-Peng He for living cells experiment.

语种：英文

外文关键词：Colorimetry - Fluorescence quenching - Metal ions

摘要：In this work, a new reversible colorimetric and fluorescent probe for sequential recognition of copper ions and biothiols is synthesized easily. Based on the chelation-enhanced fluorescence quenching (CHEQ) effect, this probe shows high sensitivity and selectivity towards Cu2+, which can be detected by the naked eye. And this experimental phenomenon can also be realised upon addition of biothiols, restoring their initial fluorescence intensity. This probe also features a very high response speed (less than 5 seconds) and a large stokes shift (178 nm) toward Cu2+ and biothiols. Moreover, the detection limit for Cu2+ and biothiols is as low as 7.34 nM and 10.3 nM, respectively. Additionally, this ON-OFF-ON-type fluorescence recognition cycle can be repeated more than 5 times by addition of Cu2+ and biothiols in turn. Particularly, this 1-Cu2+ ensemble is further successfully applied for GSH detection in living cells.