文献类型：期刊文献

中文题名：Q-Orbitrap-液相色谱串联质谱法测定鸡蛋中甲硝唑及新的代谢物

英文题名：Determination of metronidazole and new metabolites in eggs by Q-Orbitrap-liquid chromatography tandem mass spectrometry

作者：秦宇[1,2];张申平[2];王安琪[2];葛宇[2];宫衡[1]

机构：[1]华东理工大学生物工程学院,上海200237;[2]上海市质量监督检验技术研究院,上海200233

年份：2026

卷号：45

期号：3

起止页码：409

中文期刊名：分析试验室

外文期刊名：Chinese Journal of Analysis Laboratory

收录：;EI(收录号：20261720554186);北大核心:【北大核心2023】；

基金：上海市质量监督检验技术研究院项目(KY-2022-2-SP)资助。

语种：中文

中文关键词：甲硝唑;羧基甲硝唑;鸡蛋;高分辨质谱;液相色谱串联质谱法

外文关键词：metronidazole;carboxyl metronidazole;eggs;high resolution mass spectrometry;liquid chromatography tandem mass spectrometry

摘要：建立了液相色谱串联质谱法同时测定鸡蛋中甲硝唑及新代谢物的方法。通过液相色谱串联高分辨质谱进行定性扫描,在鸡蛋中发现一种新的甲硝唑代谢物-羧基甲硝唑。进一步建立了以0.1%(V/V)甲酸乙腈溶液提取,石油醚净化,Waters Atlantis T3(100 mm×2.1 mm, 3μm)柱分离,正离子扫描和多反应监测模式采集,液相色谱串联质谱法测定鸡蛋中甲硝唑及其代谢物的定量方法。结果表明,在质量浓度0.50~20.0 ng/mL范围内,甲硝唑及其代谢物呈良好的线性关系,检出限为0.25μg/kg,定量限为0.5μg/kg;平均加标回收率在85.5%~110.9%之间,相对标准偏差在0.9%~13%之间。该方法能够应用于实际鸡蛋样品中甲硝唑及代谢物的检测。
A method for the simultaneous determination of metronidazole and new metabolites in eggs by liquid chromatographytandem mass spectrometry was established in this study.A new metronidazole metabolite,carboxyl metronidazole,was found by qualitative scanning of liquid chromatography tandem high-resolution mass spectrometry.Further,a quantitative method was established with liquid chromatography tandem mass spectrometry for the determination of metronidazole and its metabolites in eggs by 0.1%(V/V)formic acid acetonitrile extraction,petroleum ether purification,Waters Atlantis T3 column(100 mm×2.1 mm,3μm)separation,positive ion scanning and multi-reaction monitoring mode collection.The results showed that metronidazole and its metabolites showed good linear relationships in the range of 0.5020.0 ng/mL.The limits of detection and quantification were 0.25μg/kg and 0.5μg/kg,respectively.The average recoveries were 85.5%110.9%,and the relative standard deviations were 0.9%13%.Additionally,this method can be applied to the detection of metronidazole and metabolites in actual eggs samples.