文献类型：期刊文献

英文题名：Molecular cloning and characterization of two novel cellulase genes from the mollusc Ampullaria crossean

作者：Guo, Rui[1];Ding, Ming[2];Zhang, Si-Liang[1];Xu, Gen-Jun[2,3];Zhao, Fu-Kun[2,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Proteom, Inst Biochem & Cell Biol, Shanghai 20031, Peoples R China;[3]Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Peoples R China

年份：2008

卷号：178

期号：2

起止页码：209

外文期刊名：JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000252483000008)】；

语种：英文

外文关键词：cellulase; endoglucanase; EG27; Ampullaria crossean; gene cloning

摘要：Cellulase genes have been reported not only from fungi, bacteria and plant, but also from some invertebrate animals. Here, two cellulase (endo-beta-1,4-glucanase, EC 3.2.1.4) genes, eg27I and eg27II, were cloned from the freshwater snail Ampullaria crossean cDNA using degenerate primers. The nucleotide sequences of the two genes shared 94.5% identity. The open reading frames of both genes consisted of 588 bp, encoding 195 amino acids. Both EG27I and EG27II belong to the glycoside hydrolase family 45, and each lacks a carbohydrate-binding module. The presence of introns demonstrated a eukaryotic origin of the EG27 gene, and, in addition, successful cloning of EG27 cDNA supported endogenous production of EG27 cellulase by Ampullaria crossean. Investigation of the EG27 cDNA from A. crossean will provide further information on GHF45 cellulases.