文献类型：期刊文献

英文题名：Simultaneous Detection of Diverse Glycoligand-Receptor Recognitions Using a Single-Excitation, Dual-Emission Graphene Composite

作者：Ji, Ding-Kun[1,2];Chen, Guo-Rong[1,2];He, Xiao-Peng[1,2];Tian, He[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2015

卷号：25

期号：23

起止页码：3483

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20151900819154);WOS:【SCI-EXPANDED(收录号:WOS:000356376600005)】；

基金：The authors thank the 973 project (2013CB733700), the National Natural Science Foundation of China (21202045), the Key Project of Shanghai Science and Technology Commission (13NM1400900), and the Fundamental Research Funds for Central Universities (222201414010).

语种：英文

外文关键词：fluorogenic; glycodye; graphene composite; lectin; simultaneous detection

摘要：Ligand-protein interactions (LPIs) are fundamental biological processes that manipulate a variety of cellular events. While multiple LPIs can occur concurrently or concertedly at a cellular interface, techniques which are able to simultaneously probe these diverse interactions remain challenging. Here, to the best of our knowledge, the first fluorogenic composite material (FCM) is developed that can probe diverse LPIs at a single biomimetic interface. We determined that two glycoligands coupled with fluorescence dyes with different emission colors can coassemble to a graphene oxide platform, producing an integrated FCM with a quenched fluorescence. The fluorescence of each glycodye is uniquely elicited upon interaction with a pairing protein (lectin) that selectively recognizes the glycoligand. Importantly, the dual emission of the FCM can be produced, on a single excitation, while both proteins exist, providing a concise means for the simultaneous probing of diverse LPIs.