文献类型：期刊文献

中文题名：基于紫外检测器的壳寡糖色谱分析与比较

英文题名：Analysis and Comparison of Chromatographic Separation of Chitooligosaccharides by Ultraviolet Detector

作者：周卫强[1,2,3];李娟[4];沈雪梅[3];彭超[3];陈博[3];晏礼明[4];赵黎明[1,2];李义[5,6];佟毅[5,6]

机构：[1]华东理工大学生物工程学院,上海200237;[2]生物反应器工程国家重点实验室,上海200237;[3]中粮营养健康研究院有限公司,北京102209;[4]中国科学院微生物研究所,北京100101;[5]中粮生物科技股份有限公司,蚌埠233010;[6]玉米深加工国家工程研究中心,长春130033

年份：2021

卷号：57

期号：8

起止页码：698

中文期刊名：理化检验（化学分册）

外文期刊名：Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

语种：中文

中文关键词：壳寡糖;紫外检测器;色谱;聚合度

外文关键词：chitooligosaccharide;ultraviolet detector;chromatography;degree of polymerization

摘要：基于氨基葡萄糖的紫外吸收特性,通过对氨基葡萄糖标准溶液在波长190~400 nm的检测,确定了壳寡糖(氨基葡萄糖分子的低聚物)的紫外检测波长为190~210 nm。比较了常用于寡糖分析的两种商品色谱柱(Shodex氨基柱和TOSOH酰胺柱)分离效果,两种色谱柱分离效果相当,但前者的出峰时间更早。基于Shodex氨基柱,拟合出紫外检测器条件下氨基葡萄糖定量计算的标准曲线,开发并验证了壳寡糖定性定量的分析方法,不同聚合度壳寡糖的分离程度和色谱峰分布规律与示差折光检测器的检测效果相当,并以此确定了壳寡糖混合标准样品的聚合度分布。方法可作为现行壳寡糖行业标准检测方法的有效补充。
Based on the ultraviolet(UV)absorption characteristics of glucosamine,the UV detection wavelength of chitooligosaccherides was optimized to 200 nm by detecting the standard solution of glucosamine at the wavelength of 190-400 nm.Two kinds of commercial chromatographic column(Shodex amino column and TOSOH amide column),commonly used in analysis of oligosaccharides,were tested to compare the separation effect of glucosamine,and the results showed that the separation resolution of two columns was similar,but the peak time of the former was earlier.Based on Shodex amino column,the standard curve of glucosamine for quantitative calculation was simulated successfully by UV detector.Lastly,a chromatographic separation method of chitooligosaccharides for qualitative and quantitative detection was verified.It was found that the resolution and the distribution peak of chitooligosaccharides with different degree of polymerization,were similar to those of differential refractive index detector.At the same time,the degree of polymerization distribution of chitooligosaccharides mixed standard sample(DP 2-7)was determined.As a conclusion,the proposed method was a valid supplement for the current standard detection method of chitooligosaccharides industry.