文献类型：期刊文献

英文题名：Identification of determinants for entering into a viable but nonculturable state in Vibrio alginolyticus by Tn-seq

作者：Cai, Jingxiao[1];Zhou, Mengqing[1];Zhang, Yuanxing[2,3];Ma, Yue[1,3,4];Zhang, Yibei[1,3];Wang, Qiyao[1,3,4]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2023

卷号：107

期号：5-6

起止页码：1813

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20230613546893);WOS:【SCI-EXPANDED(收录号:WOS:000925681900003)】；

基金：This work was supported by grants from the National Key Research and Development Program of China (2022YFE0101200); National Natural Science Foundation of China (31772893); China Agriculture Research System of MOF and MARA (CARS-47); and National Natural Science Foundation of China (32102850).

语种：英文

外文关键词：Viable but nonculturable (VBNC); Transposon insertion sequencing (Tn-seq); Nutrient-limited condition; Vibrio alginolyticus

摘要：The viable but nonculturable (VBNC) state is a dormant state of nonsporulating bacteria that enhances survival in adverse environments. Systematic genome-wide research on the genetic basis of VBNC formation is warranted. In this study, we demonstrated that the marine bacterium Vibrio alginolyticus lost culturability but remained viable and entered into the VBNC state when exposed to low nutrient concentrations for prolonged periods of time. Using transposon-insertion sequencing (Tn-seq), we identified 635 determinants governing the formation of the VBNC state, including 322 genes with defective effects on VBNC formation and 313 genes contributing to entry into the VBNC state. Tn-seq analysis revealed that genes involved in various metabolic pathways were shown to have an inhibitory effect on VBNC formation, while genes related to chemotaxis or folate biosynthesis promoted entry into the VBNC state. Moreover, the effects of these genes on the formation of VBNC were validated with the growth of deletion mutants of eight selected genes under nutrient-limited conditions. Interestingly, fleQ and pyrI were identified as essential for entry into the VBNC state, and they affected the formation of the VBNC state independent of RpoE or ToxR regulation. Collectively, these results provide new insights into the mechanism of VBNC formation.