文献类型：期刊文献

英文题名：Microbial community composition and diversity in production water of a high-temperature offshore oil reservoir assessed by DNA- and RNA-based analyses

作者：Zhou, Lei[1,2,3];Lu, Yu-Wei[2,3];Wang, Da-Wei[1,4];Zhang, Shi-Lun[1,4];Tang, En-Gao[1,4];Qi, Zhen-Zhen[2,3];Xie, Sheng-Nan[2,3];Wu, Jun[2,3];Liang, Bo[2,3];Liu, Jin-Feng[2,3];Yang, Shi-Zhong[2,3];Zhang, Jian[1,4];Gu, Ji-Dong[5];Mu, Bo-Zhong[2,3,6]

机构：[1]State Key Lab Offshore Oil Exploitat, Beijing 100028, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]China Natl Offshore Oil Corp CNOOC Res Inst Ltd, Beijing 100028, Peoples R China;[5]Guangdong Technion Israel Inst Technol, Environm Engn, 241 Daxue Rd, Shantou 515063, Guangdong, Peoples R China;[6]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：151

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20201708571574);WOS:【SCI-EXPANDED(收录号:WOS:000536129500009)】；

基金：This work was supported by Open fund of State Key Laboratory of Offshore Oil Exploitation (CCL2018RCPS0028RON).

语种：英文

外文关键词：Offshore petroleum reservoir; Microbial community; DNA- and RNA-Based; Active community; 16S rRNA sequencing

摘要：Microbial activity is ubiquitous and important in petroleum reservoir systems; however, current knowledge regarding the diversity and functions of microbial communities that are active in offshore petroleum reservoirs remains very limited. In the present work, high-throughput sequencing analysis of the 16S rRNA gene (DNA) and transcripts (RNA) was applied to respectively characterize the genomic and active microbial communities from two production wells in a high-temperature offshore oilfield in China. The results showed that the microbial community compositions in the two wells (X1 and X2) were very different. At the genus level, the dominant members in both genomic and active communities of sample X1 were Bosea, Acinetobacter, Sphingomonas, Candidatus Methanofastidiosum and Methanocalculus. Both genomic and active communities in sample X2 were mainly represented by Pseudomonas, Acinetobacter, Methanothermobacter, Methanosaeta, Candidatus Nitrosopumilus, Candidatus Methanomethylicus, Thermococcus, Candidatus Methanofastidiosum and Methanocalculus. Moreover, using an RNA-based approach, genera with a very low abundance and even undetected genera (based on a DNA approach) were shown to have increased activity. Besides the abundant active members, the comparison between the DNA- and RNA-based datasets also permitted the identification of genera that were relatively more active in the two production water samples. The existence of these active microorganisms suggested they might have potential functions associated with reservoir issues in the offshore oilfield. This study highlights the importance of assessing microbial diversity through a combination of DNA- and RNA-based analyses, and promotes a comprehensive and deep understanding of microbial compositions and their roles in offshore petroleum reservoirs.