文献类型：期刊文献

英文题名：Dehydrogenase-Catalyzed Oxidation of Furanics: Exploitation of Hemoglobin Catalytic Promiscuity

作者：Jia, Hao-Yu[1];Zong, Min-Hua[1];Yu, Hui-Lei[2];Li, Ning[1]

机构：[1]South China Univ Technol, State Key Lab Pulp & Paper Engn, Sch Food Sci & Engn, 381 Wushan Rd, Guangzhou 510640, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：10

期号：18

起止页码：3524

外文期刊名：CHEMSUSCHEM

收录：;EI(收录号：20173904208583);WOS:【SCI-EXPANDED(收录号:WOS:000411499600001)】；

基金：This research was financially supported by the State Key Laboratory of Pulp and Paper Engineering (2017C01), and the National Natural Science Foundation of China (21676103).

语种：英文

外文关键词：cofactor regeneration; enzyme catalysis; enzyme promiscuity; oxidation; platform chemicals

摘要：The catalytic promiscuity of hemoglobin (Hb) was explored for regenerating oxidized nicotinamide cofactors [NAD(P)(+)]. With H2O2 as oxidant, Hb efficiently oxidized NAD(P)H into NAD(P)(+) within 30min. The new NAD(P)(+) regeneration system was coupled with horse liver alcohol dehydrogenase (HLADH) for the oxidation of bio-based furanics such as furfural and 5-hydroxymethylfurfural (HMF). The target acids (e.g., 2,5-furandicarboxylic acid, FDCA) were prepared with moderate-to-good yields. The enzymatic regeneration method was applied in L-glutamic dehydrogenase (DH)-mediated oxidative deamination of L-glutamate and for L-lactic-DH-mediated oxidation of l-lactate, which furnished alpha-ketoglutarate and pyruvate in yields of 97% and 81%, respectively. A total turnover number (TTON) of up to approximately 5000 for cofactor and an E-factor of less than 110 were obtained in the bi-enzymatic cascade synthesis of alpha-ketoglutarate. Overall, a proof-of-concept based on catalytic promiscuity of Hb was provided for in situ regeneration of NAD(P)(+) in DH-catalyzed oxidation reactions.