文献类型：期刊文献

英文题名：Improved large-scale production of vitamin B₁₂ by Pseudomonas denitrificans with betaine feeding

作者：Li, Kun-Tai[1,2];Liu, Dong-Hong[1];Li, Yong-Liang[3];Chu, Ju[1];Wang, Yong-Hong[1];Zhuang, Ying-Ping[1];Zhang, Si-Liang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiangxi Agr Univ, Coll Biol Sci & Technol, Nanchang 330045, Peoples R China;[3]Grp Hua Rong Pharmaceut Co Ltd, Shijiazhuang Pharm, Shijiazhuang 050041, Peoples R China

年份：2008

卷号：99

期号：17

起止页码：8516

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000258738700095)】；

基金：This work was supported by National Basic Research Program (973 Program, No. 2007CB714303) and Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：betaine; large-scale production; vitamin B-12; Pseudomonas denitrificans

摘要：The strategy of betaine control for vitamin B-12 large-scale fermentation by Pseudomonas denitrificans was investigated in this paper. The results obtained in shake-flask experiments demonstrated that betaine could greatly stimulate vitamin B-12 biosynthesis but had an inhibition to cell growth. Based on the influence of betaine on the fermentation of P. denitrificans, betaine feeding was a beneficial strategy to solve the inconsistency between cell growth and vitamin B-12 production. As a result, an effective and economical strategy of betaine feeding was established for vitamin B-12 fermentation in 120-m(3) fermenter, in which betaine was continuously fed to maintain betaine concentration of the broth at the range of 5-7 g/l during 50-140 h of fermentation. (c) 2008 Published by Elsevier Ltd.