文献类型：期刊文献

英文题名：The effects of ROS-mediating oxygen tension on human CD34+CD38- cells induced into mature dendritic cells

作者：Fan, Jinli[1,3]; Cai, Haibo[1]; Li, Qunliang[2]; Du, Zheng[1]; Tan, Wensong[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China; [2] School of Chemistry and Chemical Engineering, Guangxi University, Nanning, China; [3] Shanghai Clinical Research Center, Shanghai, China

年份：2012

卷号：158

期号：3

起止页码：104

外文期刊名：Journal of Biotechnology

收录：EI(收录号：20121214872889)

语种：英文

外文关键词：Amino acids - Cytology - Stem cells - Cell membranes - Oxygen - Gene expression

摘要：Oxygen tension regulates the biological characteristics of hematopoietic stem and progenitor cells (HSPCs) by modulating intracellular reactive oxygen species (ROS). To better understand oxygen tension mechanism on HSPCs culture, gene expression analysis of human CD34+CD38- HSPCs was performed using microarrays. The CD34+CD38- HSPCs cultured under normoxia, hypoxia, or with N-acetyl cysteine (NAC, an ROS scavenger) were isolated for transcriptional profilings. Compared to normoxia group, 1 gene was up-regulated and 22 genes were down-regulated in hypoxia group, while 1 gene was up-regulated and 29 genes were down-regulated in NAC group. These differently expressed genes were involved in cell surface markers, blood activation and differentiation. The common down-regulated genes related to dendritic cells (DCs) maturation (CD80, CD86, and JAG1) were confirmed by real-time RT-PCR. Furthermore, the analysis of the phenotypes of DCs, including the DC-characteristic surface molecule CD1a, the costimulatory molecules CD80 and CD86, and HLA-DR, associated with the capacity of DCs to stimulate allogeneic T cells, showed that hypoxia-mediating ROS inhibited the potential of CD34+CD38- HSPCs differentiating to mature DCs. All these results demonstrated that hypoxia-reducing ROS down-regulated the genes driving CD34+CD38- HSPCs differentiation, which provides an interesting molecular hint to direct their development to DCs during cultures. ? 2012 Elsevier B.V.