文献类型：期刊文献

英文题名：An improved pretreatment method for efficient detection of antibiotic residues in aquatic products by LC-MS/MS

作者：Qin, Yu[1];Zhang, Shenping[2];Shi, Jingwen[2];Gong, Heng[1]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[2]Shanghai Inst Qual Inspect & Tech Res, Shanghai 200233, Peoples R China

年份：2025

卷号：266

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001658123900001)】；

基金：This work was supported by Project of Shanghai Institute of Quality Supervision and Inspection Technology (KY-2022-2-SP).

语种：英文

外文关键词：Antibiotics residues; Two-step extraction; Captiva EMR-Lipid; LC-MS/MS; Aquatic products

摘要：Antibiotics are a class of compounds that are effective against bacterial infections and are widely used in aquaculture. Herein, a method based on high performance liquid chromatography coupled with tandem mass spectrometry was developed to detect nine classes of 116 antibiotics in aquatic products. The antibiotics were extracted in two steps using 1 % formic acid-acetonitrile with 0.1 mol/L Na(2)EDTA-Mcllvain buffer solution and acetonitrile, purified using Captiva EMR-Lipid, and separated with an ACQUITY UPLC (R) HSS T-3 column (100 mm x 2.1 mm, 3.5 mu m) using acetonitrile and 0.1 % formic acid with 5 mM ammonium acetate as mobile phase for gradient elution. Calibration curve from 0.10 to 100 ng/mL exhibited good correlation coefficient (R-2 >0.99) for quantification. The analytical performance of the developed method was satisfactory, with limits of quantification = 5.00 ng/g for detecting of antibiotics in aquatic products. The recoveries of the 116 antibiotics ranged from 60.57 % to 127.33 %, with precision (expressed as relative standard deviation) of < 30.20 %. All parameters showed acceptable values and conformed to the Commission Decision 2021/808/EC criteria. When compared with other techniques, the developed method meets the quantitative requirements of highthroughput analysis and can simultaneously analyze 116 antibiotics in aquatic products.