文献类型：期刊文献

英文题名：Phosphate effect on production of ginseng saponin and polysaccharide by cell suspension cultures of Panax ginseng and Panax quinquefolium

作者：Liu, S; Zhong, JJ

机构：[1]E China Univ Sci & Technol, Coll Biotechnol, Biochem Engn Res Inst, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Natl Engn Res Ctr Biotechnol Shanghai, Shanghai 200237, Peoples R China

年份：1998

卷号：33

期号：1

起止页码：69

外文期刊名：PROCESS BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000072139200010)】；

语种：英文

外文关键词：ginseng saponin; ginseng polysaccharide; initial phosphate concentration; Panax ginseng; Panax quinquefolium; plant cell culture; useful metabolite production

摘要：The effects of inorganic phosphate on cell growth, accumulation of ginseng saponin (secondary metabolite) and ginseng polysaccharide (primary metabolite), together with nutrient utilisation, were investigated in suspension cultures of Panax ginseng (PG) and Panax quinquefolium strain Q91625 (PQ). The results indicate that the crucial concentration of phosphate for cell growth of PG and PQ was 1.04 and 0.65 mM, respectively. Bound phosphorus content for PG and PQ cells was determined to be 4 and 7 mg P/g DW, respectively, which suggested a more urgent demand of phosphate during cell cultivation of PQ. For simultaneous production of ginseng saponin and polysaccharide, the optimal concentration of phosphate in PG cell cultures was 0.42 mM, and saponin and polysaccharide accumulation was 643 mg litre(-1) and 2.18 g litre(-1), respectively; 1.25 mM of medium phosphate was the best for biosyntheses of saponin (960 mg litre(-1)) and polysaccharide (1.8 g litre(-1)) by PQ cell cultures. (C) 1998 Elsevier Science Ltd.