文献类型：期刊文献

中文题名：Enzyme-Triggered Fluorescence Turn-on： A Probe for Specifically Imaging Ovarian-Cancer-Related γ-Glutamyltranspeptidase

英文题名：Enzyme-Triggered Fluorescence Turn-on： A Probe for Specifically Imaging Ovarian-Cancer-Related γ-Glutamyltranspeptidase

作者：Jie Tian[1];Qinglong Yan[2];Ying Zhu[2];Jichao Zhang[2];Jiao Li[1];Ben Shi[1];Ge Xu[1];Chunhai Fan[2];Chunchang Zhao[1]

机构：[1]Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, China;[2]Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laborato- ry of lnterfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

年份：2017

卷号：35

期号：11

起止页码：1711

中文期刊名：Chinese Journal of Chemistry

外文期刊名：中国化学（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2017_2018】；

基金：We gratefully acknowledge the financial support by the National Natural Science Foundation of China （Nos. 21372083. 21672062）.

语种：英文

中文关键词：fluorescence turn-on;GGT;the aromatic hydrocarbon transfer;living cells;specifically targeting

外文关键词：fluorescence turn-on, GGT, the aromatic hydrocarbon transfer, living cells, specifically targeting

摘要：A fluorescent turn-on probe for specifically targeting γ-glutamyltranspeptidase （GGT） was designed and synthe- sized by integrating boron-dipyrromethene （BODIPY） as a chromophore and glutathione （GSH） as the GGT sub- strate. GGT-catalyzed the cleavage of the γ-glutamyl bond and generated the aromatic hydrocarbon transfer between the sulfur and the nitrogen atom in BODIPY, leading to distinct optical changes. Such specific responsiveness pro- vides an easily distinguishable fluorescence signal to visualize the GGT activity in living cells and differentiate GGT-positive cancer cells from GGT-negative cells.
A fluorescent turn-on probe for specifically targeting γ-glutamyltranspeptidase （GGT） was designed and synthe- sized by integrating boron-dipyrromethene （BODIPY） as a chromophore and glutathione （GSH） as the GGT sub- strate. GGT-catalyzed the cleavage of the γ-glutamyl bond and generated the aromatic hydrocarbon transfer between the sulfur and the nitrogen atom in BODIPY, leading to distinct optical changes. Such specific responsiveness pro- vides an easily distinguishable fluorescence signal to visualize the GGT activity in living cells and differentiate GGT-positive cancer cells from GGT-negative cells.