文献类型：期刊文献

英文题名：A glycoconjugate-based gold nanoparticle approach for the targeted treatment of Pseudomonas aeruginosa biofilms

作者：Zhang, Chao[1];Shi, De-Tai[2,3,4];Yan, Kai-Cheng[2,3,5];Sedgwick, Adam C.[6];Chen, Guo-Rong[2,3];He, Xiao-Peng[2,3];James, Tony D.[5];Ye, Bing[1];Hu, Xi-Le[2,3];Chen, Daijie[7]

机构：[1]Jinan Childrens Hosp, Emergency Dept, 23976 Jingshi Rd, Jinan, Shandong, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Key Lab Adv Mat, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Joint Int Res Lab Precis Chem & Mol Engn, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Jingdezhen Ceram Inst, Sch Mat Sci & Engn, Jingdezhen 333403, Peoples R China;[5]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England;[6]Univ Texas Austin, Dept Chem, 105 E 24th St A5300, Austin, TX 78712 USA;[7]Shanghai Jiao Tong Univ, Sch Pharm, 800 Dongchuan Rd, Shanghai 200240, Peoples R China

年份：2020

卷号：12

期号：45

起止页码：23234

外文期刊名：NANOSCALE

收录：;EI(收录号：20205009598120);WOS:【SCI-EXPANDED(收录号:WOS:000593021300037)】；

基金：The authors thank the NSFC (no. 91853201 and 21907030), the Shanghai Municipal Science and Technology Major Project (2018SHZDZX03), the National Key Sci-Tech Special Projects of Infection Diseases of China (2018ZX10732202), the International Cooperation Program of Shanghai Science and Technology Committee (17520750100) and the China Postdoctoral Science Foundation (2018M630407). TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award.

语种：英文

外文关键词：Carbohydrates - Targeted drug delivery - Controlled drug delivery - Gold nanoparticles - Metal nanoparticles - Proteins - Fluorescence imaging - Fluorophores

摘要：In this study, "core-shell" gold nanoparticles (AuNPs) have been functionalised using a simple one-pot approach to form fucose-based glycoconjugate AuNPs (Fuc-AuNPs) and galactose-based glycoconjugate AuNPs (Gal-AuNPs), respectively. Owing to the selective carbohydrate-based recognition of the key virulence factors of P. aeruginosa, LecB (fucose-specific lectin)/LecA (galactose-specific lectin), Fuc-AuNPs and Gal-AuNPs-based imaging and therapeutic strategies were evaluated towards P. aeruginosa. Both Fuc-AuNPs and Gal-AuNPs were non-covalently loaded with the fluorophore dicyanomethylene 4H-pyran (DCM) to afford two highly selective fluorescence imaging agents for the visualisation of P. aeruginosa. The loading of Fuc-AuNPs and Gal-AuNPs with the known antibiotic Ceftazidime (CAZ) exhibited an enhanced therapeutic effect, illustrating the significance of this targeted drug delivery strategy. Exploiting the phototherapeutic properties of AuNPs, photoirradiation (600 nm) of Fuc-AuNP@CAZ/Gal-AuNP@CAZ provided both photothermal and photodynamic therapeutic (PTT/PDT) effects, which facilitated the release of CAZ. Fuc-AuNP@CAZ and Gal-AuNP@CAZ were shown to be effective photo/chemotherapeutics resulting in almost complete eradication of P. aeruginosa biofilms formed on clinically relevant surfaces (glass slides and steel surface).