文献类型：期刊文献

英文题名：Human serum albumin-based supramolecular host-guest boronate probe for enhanced peroxynitrite sensing

作者：Tian Jr, He[1,2];Guo, Chen[1,2];Hu, Xi-Le[1,2];Wang, Jing-Bo[1,2];Zang, Yi[4,7];James, Tony D. D.[5,6];Li, Jia[4];He, Xiao-Peng[1,2,3]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Int Cooperat Lab Signal Transduct, Natl Ctr Liver Canc, Shanghai 200438, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China;[5]Univ Bath, Dept Chem, Bath BA2 7AY, England;[6]Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China;[7]Lingang Lab, Shanghai 201203, Peoples R China

年份：2023

卷号：21

期号：22

起止页码：4661

外文期刊名：ORGANIC & BIOMOLECULAR CHEMISTRY

收录：;EI(收录号：20232214171500);WOS:【SCI-EXPANDED(收录号:WOS:000991907300001)】；

基金：The authors thank the National Natural Science Foundation of China (NSFC) (No. 92253306 and 82130099), the Shanghai Municipal Science and Technology Major Project (No. 2018SHZDZX03), the Fundamental Research Funds for the Central Universities (222201717003), the Programme of Introducing Talents of Discipline to Universities (B16017) and Open Funding Project of the State Key Laboratory of Bioreactor Engineering of East China University of Science and Technology for financial support. The Research Center of Analysis and Test of East China University of Science and Technology is gratefully acknowledged for assistance in analytical experiments. TDJ wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support.

语种：英文

外文关键词：Fluorescence - Supramolecular chemistry - X ray scattering

摘要：Peroxynitrite (ONOO-) is an important oxygen/nitrogen reactive species implicated in a number of physiological and pathological processes. However, due to the complexity of the cellular micro-environment, the sensitive and accurate detection of ONOO- remains a challenging task. Here, we developed a long-wavelength fluorescent probe based on the conjugation between a TCF scaffold and phenylboronate; the resulting conjugate is capable of supramolecular host-guest assembly with human serum albumin (HSA) for the fluorogenic sensing of ONOO-. The probe exhibited an enhanced fluorescence over a low concentration range of ONOO- (0-9.6 mu M), whist the fluorescence was quenched when the concentration of ONOO- exceeded 9.6 mu M. In addition, when human serum albumin (HSA) was added, the initial fluorescence of the probe was significantly enhanced, which enabled the more sensitive detection of low-concentrations of ONOO- in aqueous buffer solution and in cells. The molecular structure of the supramolecular host-guest ensemble was determined using small-angle X-ray scattering.