文献类型：期刊文献

中文题名：双硒葡聚糖水凝胶的合成及其药物释放行为

英文题名：Synthesis and Drug Release Behavior of Diselenide Dextran Hydrogel

作者：程鹏辉[1];刘小云[1];庄启昕[1]

机构：[1]华东理工大学材料科学与工程学院特种功能高分子材料及相关技术教育部重点实验室,上海200237

年份：2019

卷号：32

期号：1

起止页码：103

中文期刊名：功能高分子学报

外文期刊名：Journal of Functional Polymers

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

语种：中文

中文关键词：双硒键;葡聚糖;水凝胶;氧化响应;药物释放

外文关键词：diselenide bond;dextran;hydrogel;oxidation response;drug release

摘要：首先,通过双硒化钠(Na2Se2)与11-溴-1-十一醇的亲核取代反应合成了一种二羟基二硒化物;然后,通过该二硒化物与异佛尔酮二异氰酸酯(IPDI)反应合成了一种异氰酸根封端的双硒交联剂;最后,通过葡聚糖与该交联剂的反应合成了一种含双硒键的葡聚糖水凝胶。采用核磁共振氢谱(~1H-NMR)、傅里叶红外光谱(FT-IR)、凝胶渗透色谱(GPC)、拉曼光谱(Raman)、旋转流变仪(Rheology)、紫外-可见分光光度计(UV-Vis)等表征方法研究了产物的结构、溶胀性能、氧化响应行为、流变性能和体外药物释放行为。结果表明:含双硒键的葡聚糖水凝胶具有氧化响应能力;在没有氧化刺激条件下,该水凝胶24h的累积药物释放量为45%左右,而在氧化刺激条件下(w(H_2O_2)=0.2),只需14h便可释放90%的负载药物。
The reactive oxygen species(ROS),like hydrogen peroxide(H2O2),generated commonly during metabolism,and the increased level of H2O2 in tumor cells could be up to 0.5nmol/(104 cells·h)in comparison with that in normal cells,which provided a possibility for the usage of oxidation-responsive hydrogels in biomedical field.Herein,di-(1-hydroxylundecyl)diselenide was prepared by the nucleophilic substitution reaction between sodium diselenide(Na2Se2) and 11-bromoundecanol,and then reacted with isophorone diisocyanate(IPDI)into an isocyanate-terminated diselenide crosslinker.Finally,a diselenide dextran hydrogel was synthesized by crosslinking dextran with the diselenide agent.The structure,swelling profile,oxidation-responsive capability,rheology property,and in vitro drug release behavior of the diselenide dextran hydrogel were characterized by proton nuclear magnetic resonance(1 H-NMR)spectrum,Fourier-transform infrared(FT-IR)spectra,gel permeation chromatography(GPC),Raman spectrum,rotational rheometer,and ultraviolet-visible(UV-Vis) spectrophotometry.Results demonstrated that the hydrogel with a lower crosslinking degree showed a higher swelling ratio.In addition,the dissolution of hydrogel was observed visually after adding H2O2 solution due to the decomposition of diselenide bonds in the polymer network,indicating that the resultant hydrogel could be in response to the oxidation stimulus.Furthermore,in vitro drug release experiments were carried out by using rhodamin B(RhB)as a model agent.The results revealed that the hydrogel could only release 45%of the loaded drug in 24 hwithout H2O2,while 90%of the loaded drug could be released in 14 hunder an oxidizing environment(i.e.,w(H2O2)=0.2%),indicating that the diselenide dextran hydrogel was a promising candidate for drug release.