文献类型：期刊文献

中文题名：Logically Sensing Aggregate Process and Discriminating SDS from Other Surfactants with the Assistance of BSA

英文题名：Logically Sensing Aggregate Process and Discriminating SDS from Other Surfactants with the Assistance of BSA

作者：Qian Junhong[1];Xu Yufang[2];Qian Xuhong[2]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2012

卷号：30

期号：6

起止页码：1283

中文期刊名：Chinese Journal of Chemistry

外文期刊名：CHINESE JOURNAL OF CHEMISTRY

收录：;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000305389200016)】；CSCD:【CSCD2011_2012】；

基金：This work was supported by the National Basic Research 973 Program (No. 2011CB910403), Open Project Fund of Shanghai Key Laboratory of Scene Material Evidence (No. 2011XCWZK02) and the Research Fund for Outstanding Young Teachers in ECUST (No. YJ0157145). We thank Prof. Albert M. Brouwer (Van't Hoff Institute for Molecular Sciences, University of Amsterdam) for the improvement of this paper.

语种：英文

中文关键词：fluorescent sensor;micelle;sodium dodecyl sulfate （SDS）;bovine serum albumine （BSA）;adding or-der

外文关键词：fluorescent sensor; micelle; sodium dodecyl sulfate (SDS); bovine serum albumine (BSA); adding order

摘要：An amphiphilic fluorescent probe, 3-dodecylamino dihydrogen imidazo[2,l-a]benz[de]isoquinolin-7-one （compound 3）, was used to sense the aggregate formation process of bovine serum albumine （BSA）, sodium dode- cyl sulfate （SDS） and their mixed system. The fluorescence intensity of 3 was significantly affected by the adding order of SDS and BSA, and SDS can be distinguished from other surfactants with the aid of BSA, but only when 3 is allowed to interact with BSA first. The results revealed that compound 3 is preferentially sited in the hydrophobic region of BSA, and thermodynamically in SDS-BSA mixed aggregate. Sodium phosphate buffer solution （PBS） and BSA played important but distinct roles in distinguishing SDS micelle from the others.
An amphiphilic fluorescent probe, 3-dodecylamino dihydrogen imidazo[2,1-a]benz[de]isoquinolin-7-one (compound 3), was used to sense the aggregate formation process of bovine serum albumine (BSA), sodium dodecyl sulfate (SDS) and their mixed system. The fluorescence intensity of 3 was significantly affected by the adding order of SDS and BSA, and SDS can be distinguished from other surfactants with the aid of BSA, but only when 3 is allowed to interact with BSA first. The results revealed that compound 3 is preferentially sited in the hydrophobic region of BSA, and thermodynamically in SDS-BSA mixed aggregate. Sodium phosphate buffer solution (PBS) and BSA played important but distinct roles in distinguishing SDS micelle from the others.