文献类型：期刊文献

英文题名：Protein separation by sequential selective complex coacervation

作者：Zhou, Jin[1,2];Cai, Ying[1,2];Wan, Yuting[1,2];Wu, Bohang[1,2];Liu, Jinbo[1,2];Zhang, Xinxin[1,2];Hu, Weiwei[3];Stuart, Martien A. Cohen[1,2];Wang, Junyou[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Chem Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Multiphase Mat Chem Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Shanghai Clin Res Ctr Bone Dis, Dept Osteoporosis & Bone Dis, Shanghai Peoples Hosp 6,Sch Med, Shanghai 200233, Peoples R China

年份：2023

卷号：650

起止页码：2065

外文期刊名：JOURNAL OF COLLOID AND INTERFACE SCIENCE

收录：;EI(收录号：20232614321081);WOS:【SCI-EXPANDED(收录号:WOS:001057949600001)】；

基金：This work was financially supported by 1000 Foreign Experts Program (WQ20163100341) and the National Natural Science Foundation of China (82170895) .

语种：英文

外文关键词：Polyelectrolytes; Complex coacervation; Selective complexation; Protein separation

摘要：In food manufacturing and particular biomedical products selected proteins are often required. Obtaining the desired proteins in a pure form from natural resources is therefore important, but often very challenging. Herein, we design a sequential coacervation process that allows to efficiently isolate and purify proteins with different isoelectric points (pIs) from a mixed solution, namely Bovine Serum Albumin (BSA, pI = 4.9) and Peroxidase from Horseradish (HRP, pI = 7.2). The key to separation is introducing a suitable polyelectrolyte that causes selective complex coacervation at appropriate pH and ionic strength. Specifically, polyethyleneimine (PEI), when added into the mixture at pH 6.0, produces a coacervation which exclusively contains BSA, leading to a supernatant solution containing 100 % HRP with a purity of 91 %. After separating the dilute and dense phases, BSA is recovered by adding poly(acrylic acid) (PAA) to the concentrated phase, which displaces BSA from the complex because it interacts more strongly with PEI. The supernatant phase after this step contains approximately 75 % of the initial amount of BSA with a purity of 99 %. Our results confirm that coacervation under well defined conditions can be selective, enabling separation of proteins with adequate purity. Therefore, the established approach demonstrates a facile , sustainable strategy with potential for protein separation at industrial scale.