文献类型：期刊文献

英文题名：Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis

作者：Xiong, Liang-Bin[1,2,3];Liu, Hao-Hao[2];Song, Lu[2];Dong, Miao-Miao[2];Ke, Jie[2];Liu, Yong-Jun[2];Liu, Ke[2];Zhao, Ming[2];Wang, Feng-Qing[2,3];Wei, Dong-Zhi[2]

机构：[1]Shanghai Univ Med & Hlth Sci, Jiading Dist Cent Hosp, Shanghai 201800, Peoples R China;[2]East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Huawei Safety Evaluat & Med Res Shanghai Co Ltd, Shanghai 201206, Peoples R China

年份：2022

卷号：7

期号：1

起止页码：453

外文期刊名：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000763657800001)】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 21776075 and 32100067), the Natural Science Foundation of Shanghai (No. 20ZR1415100), the National Key Research and Development Program of China (No. SQ2020YFC210061), the China Postdoctoral Science Foundation (No. 2020M671028), the Shanghai Municipal Health Commission (No. 20204Y0380), the Teacher's Professional Development Project of Shanghai Municipal Education Commission, and the Scientific Research Foundation of SUMHS.

语种：英文

外文关键词：Mycolicibacterium; 9-OHAD; Cell permeability; Soybean phytosterol; fbpC3; embC

摘要：Biotransformation of soybean phytosterols into 9 alpha-hydroxy-4-androstene-3,17-dione (9-OHAD) by mycobacteria is the core step in the synthesis of adrenocortical hormone. However, the low permeability of the dense cell envelope largely inhibits the overall conversion efficiency of phytosterols. The antigen 85 (Ag85) complex encoded by fbpA, fbpB, and fbpC was proposed as the key factor in the combined catalysis of mycoloyl for producing mycolyl-arabinogalactan (m-AG) and trehalose dimycolate (TDM) in mycobacterial cell envelope. Herein, we confirmed that fbpC3 was essential for the biotransformation of trehalose monomycolate (TMM) to TDM in Mycolicibacterium neoaurum. The deficiency of this gene raised the cell permeability, thereby enhancing the steroid uptake and utilization. The 9-OHAD yield in the fbpC3-deficient 9-OHAD-producing strain was increased by 21.3%. Moreover, the combined deletion of fbpC3 and embC further increased the 9-OHAD yield compared to the single deletion of fbpC3. Finally, after 96 h of bioconversion in industrial resting cells, the 9OHAD yield of 11.2 g/L was achieved from 20 g/L phytosterols and the productivity reached 0.116 g/L/h. In summary, this study suggested the critical role of the fbpC3 gene in the synthesis of TDM in M. neoaurum and verified the feasibility of improving the bioconversion efficiency of phytosterols through the cell envelope engineering strategy.