文献类型：期刊文献

英文题名：Significant decrease of broth viscosity and glucose consumption in erythromycin fermentation by dynamic regulation of ammonium sulfate and phosphate

作者：Chen, Yong[1,2];Wang, Zejian[1];Chu, Ju[1];Zhuang, Yingping[1];Zhang, Siliang[1];Yu, Xiaoguang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Qingdao Agr Univ, Food Sci & Engn Coll, Qingdao 266109, Shandong, Peoples R China

年份：2013

卷号：134

起止页码：173

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20131316149906);WOS:【SCI-EXPANDED(收录号:WOS:000318052200011)】；

基金：This work was financially supported by a Grant from the Major State Basic Research Development Program of China (973 Program, No. 2012CB721006), National Natural Science Foundation of China (No. 21276081), the National Scientific and Technological Major Special Project (Significant Creation of New drugs, No. 2011ZX09203-001-03), and Research Fund for the Doctoral Program of Higher Education of China (No. 20110074110015).

语种：英文

外文关键词：Erythromycin; Broth viscosity; Glucose consumption; Ammonium sulfate; Medium modification

摘要：In this study, the effects of nitrogen sources on broth viscosity and glucose consumption in erythromycin fermentation were investigated. By controlling ammonium sulfate concentration, broth viscosity and glucose consumption were decreased by 18.2% and 61.6%, respectively, whereas erythromycin biosynthesis was little affected. Furthermore, erythromycin A production was increased by 8.7% still with characteristics of low broth viscosity and glucose consumption through the rational regulations of phosphate salt, soybean meal and ammonium sulfate. It was found that ammonium sulfate could effectively control proteinase activity, which was correlated with the utilization of soybean meal as well as cell growth. The pollets formation contributed much to the decrease of broth viscosity. The accumulation of extracellular propionate and succinate under the new regulation strategy indicated that higher propanol consumption might increase the concentration of methylmalonyl-CoA and propionyl-CoA and thus could increase the flux leading to erythromycin A. (C) 2013 Elsevier Ltd. All rights reserved.