文献类型：期刊文献

英文题名：Genome Mining and Heterologous Expression-Guided Discovery of Mangicol Sesterterpenoids with Antimicrobial and Antineuroinflammatory Activities

作者：Xing, Cuiping[1];Yang, Huanting[1];Lv, Kangjie[1];Lan, Keying[1];Zhu, Guoliang[1];Zhu, Bin[1];Ren, Biao[2,3];Jiang, Yi[4];Jiang, Chenglin[4];Dai, Huanqin[5];Mo, Xuming[6];Hsiang, Tom[7];Abdelmageed, Wael M.[8];Zhang, Lixin[1];Jiang, Lan[6];Liu, Xueting[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China;[3]Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu 610041, Peoples R China;[4]Yunnan Univ, Yunnan Inst Microbiol, Key Lab Res & Dev Actinomycete Resources, Kunming 650500, Peoples R China;[5]Chinese Acad Sci, Key Lab Pathogen Microbiol & Immunol, Inst Microbiol, Beijing 100101, Peoples R China;[6]Nanjing Med Univ, Dept Cardiothorac Surg, Childrens Hosp, Nanjing 210093, Peoples R China;[7]Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada;[8]Assiut Univ, Fac Pharm, Dept Pharmacognosy, Assiut 71526, Egypt

年份：2025

卷号：73

期号：31

起止页码：19474

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20253419010196);WOS:【SCI-EXPANDED(收录号:WOS:001535141500001)】；

基金：We acknowledge the generous funding received from the Shanghai Sci-Tech Inno Center for Infection & Immunity (SSIII-2024A01 and SSIII-2024A02), Shanghai Municipal Science and Technology Major Project, National Key Research and Development Program of China (2020YFA090032 and 2019YFA0906200), National Natural Science Foundation of China (21977029 and 81903529), Open Project Funding of the State Key Laboratory of Bioreactor Engineering, and 111 Project (B18022). We are grateful to Prof. Hideaki Oikawa (Hokkaido University) for his kind guidance. The genome of strain FO14005 was sequenced and assembled with financial support from the Natural Sciences and Engineering Research Council of Canada, awarded to Prof. T. Hsiang.

语种：英文

外文关键词：phytopathogenic fungus; sesterterpenoid; heterologousexpression; mangicol; FBMN; antibacterialactivity; antineuroinflammatory

摘要：The accelerating increase in antimicrobial resistance presents serious dangers to global health, food safety, and agricultural productivity. In this study, we explored fungal-derived sesterterpenoids as potential antimicrobial agents, focusing on mangicols biosynthesized by a newly identified bifunctional terpene synthase from Fusarium oxysporum 14005. By combination of genome mining, heterologous pathway reconstitution, and feature-based molecular networking, eight new mangicols featuring epoxy and tetrahydrofuran moieties on their side chains were identified and characterized. Antimicrobial assays revealed that several of these derivatives exhibited potent activity against Ralstonia solanacearum, Staphylococcus aureus, and Streptococcus mutans, with minimum inhibitory concentrations ranging from 6.25 to 25 mu M. Furthermore, compounds 3-5 demonstrated significant antineuroinflammatory effects in murine BV2 microglial cells with 50.0, 53.13, and 48.05%, respectively. The results offer mechanistic insights into the biosynthesis and bioactivity of mangicol-type sesterterpenoids and support their potential as novel antimicrobial and anti-inflammatory agents for addressing antimicrobial resistance.