文献类型：期刊文献

英文题名：Chondroitin sulfate-polydopamine modified polyethylene terephthalate with extracellular matrix-mimetic immunoregulatory functions for osseointegration

作者：Li, Ya-Min[1];Wu, Jing-Yao[2];Jiang, Jia[1];Dong, Shi-Kui[1];Chen, Yun-Su[3];He, Hong-Yan[2];Liu, Chang-Sheng[2];Zhao, Jin-Zhong[1]

机构：[1]Shanghai Jiaotong Univ Affiliated Peoples Hops 6, Dept Sports Med, 600 Yishan Rd, Shanghai 200233, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China;[3]Shanghai Jiaotong Univ Affiliated Peoples Hosp 6, Dept Joint Surg, 600 Yishan Rd, Shanghai 200233, Peoples R China

年份：2019

卷号：7

期号：48

起止页码：7756

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20195207903512);WOS:【SCI-EXPANDED(收录号:WOS:000502339700010)】；

基金：This study was supported by the National Key Research and Development Program of China (Grant No. 2018YFC1106200 and 2018YFC1106202), the National Natural Science Foundation of China (Grant No. 81871753, 81772341, and 31570971), the Shanghai International S&T Cooperation Project (Grant No. 18520710100), the New Frontier Multi-center Project of Shenkang Hospital Development Center of Shanghai (Grant No. SHDC12017121), the Collaborative Innovation Transformation Project of Shanghai Jiao Tong University School of Medicine (Grant No. TM201726) and the Doctor Innovation Project of Shanghai Jiao Tong University School of Medicine (BXJ201839).

语种：英文

外文关键词：Biomimetics - Plastic bottles - Grafting (chemical) - Stem cells - Sulfur compounds - Cytology - Bone

摘要：Optimal integration between the polyethylene terephthalate (PET) graft and host bone is a prerequisite to obtain a satisfactory outcome after graft implantation for ligament reconstruction. Recent studies indicate that complex biosignals including immunoregulation, cell recruitment, and osteogenic differentiation provided by the extracellular matrix (ECM) are conducive to promoting osseointegration. In the present study, a chondroitin sulfate (CS)/polydopamine-modified PET graft was developed to regulate the local immune microenvironment, guide stem cell behavior, and promote new bone formation. We found that CS-modified PET grafts significantly regulated the macrophage phenotype switching from M1 to M2 and promoted the expression of pro-repair cytokines including interleukin (IL)-4, IL-10 and transforming growth factor (TGF)-beta 1. Moreover, the immunoregulatory function of CS-modified PET guided stem cell behaviors, including recruitment, adhesion, and proliferation, and enhanced the osteogenic differentiation of stem cells. In vivo experiments confirmed that CS-modified PET switched the local immune microenvironment status from pro-inflammatory to anti-inflammatory, up-regulated osteogenic marker expression, and promoted the bone regeneration process, so as to achieve graft-bone osseointegration. These results indicate that an ECM-biomimetic immunoregulatory coating is an effective approach to promote graft integration. This study proposes an effective strategy for an artificial graft to achieve graft-bone osseointegration through immunoregulatory osteogenesis.