文献类型：期刊文献

中文题名：贴壁培养方式中昆虫细胞的生长限制性因素研究

英文题名：Identification of Growth limiting Factors for Insect Cells Grown in Stationary Cultures

作者：赵佼[1];周燕[1];谭文松[1];俞俊棠[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：1999

卷号：15

期号：3

起止页码：383

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：昆虫细胞;贴壁培养;环境因素;生长性能;代谢参数

外文关键词：Insect cells, stationary cultures, environmental factors,growth properties,metabolic rates

摘要：以昆虫细胞为宿主生产病毒杀虫剂或进行基因工程产品的开发，是动物细胞培养领域十分有吸引力的研究方向。近年来，昆虫细胞的体外培养尽管在培养条件的优化［１］、培养基的开发［２］以及工艺流程的设计［３］等诸多方面取得了令人鼓舞的进展，但由于对影响细胞生长及代...
An investigation was undertaken into the growth properties of two different insect cell lines grown in stationary cultures.The environmental factors which could affect the growth of attached cells were identified and a significant increase in density of attached cells were obtained by optimization of several environmental factors.In addition to the identification of environmental factors,the study also went deep into the growth characteristics inherent in different cell lines.It was shown that BTI Tn 5B1 4 cells were stricter with culture conditions as compared with IPLB Sf21 AE cells.Under identical culture conditions,BTI Tn 5B1 4 cells had both lower growth rate and shorter exponential growth phase than IPLB Sf21 AE cells.Further analysis of metabolic rates revealed that higher glucose utilization rate but lower yield factor was typical of BTI Tn 5B1 4 cells.