文献类型：期刊文献

英文题名：Fermentative Production of Phenolic Glucosides by Escherichia coli with an Engineered Glucosyltransferase from Rhodiola sachalinensis

作者：He, Qinglin[1,2];Yin, Hua[2,3];Jiang, Jingjie[4];Bai, Yanfen[2,3];Chen, Ning[1];Liu, Shaowei[4];Zhuang, Yibin[2,3];Liu, Tao[2,3]

机构：[1]Tianjin Univ Sci & Technol, Coll Biotechnol, Natl & Local United Engn Lab Metab Control Fermen, Tianjin 300457, Peoples R China;[2]Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China;[3]Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China;[4]East China Univ Sci & Technol, Coll Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：65

期号：23

起止页码：4691

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20172503795509);WOS:【SCI-EXPANDED(收录号:WOS:000403631100018)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (no. 21302214), the 973 Program of China (no. 2012CB721100), and the Biological Resources Service Plan of CAS (no. ZSTH-023).

语种：英文

外文关键词：biotraasformations; UGT73B6(FS); E. coli phenolic glucosides

摘要：Three rosmarinic acid analogs produced by recombinant Escherichia coli, two xanthones from fungi and honokiol from plants, were explored as the substrates of E. coli harboring a glucosyltransferase mutant UGT73B6(FS) to generate phenolic glucosides. Six new and two known compounds were isolated from the fermentation broth of the recombinant strain of the feeding experiments, and the compounds were identified by spectroscopy, The biotransformation of rosmarinic acid analogs and 'xanthones into corresponding glucosides was presented for the first time This study not only demonstrated the substrate flexibility of the glucosyltransferase mutant UGT73B6(FS) toward aromatic alcohols :but also provided an effective and economical method to produce phenolic glucosides by fermentation circumventing: the use of expensive precursor UDP-glucose.