文献类型：期刊文献

英文题名：Carbon-negative bio-production of short-chain carboxylic acids (SCCAs) from syngas via the sequential two-stage bioprocess by Moorella thermoacetica and metabolically engineered Escherichia coli

作者：Fei, Peng[1];Zhang, Wenrui[1];Shang, Yanzhe[2];Hu, Peng[3];Gu, Yang[4];Luo, Yuanchan[1];Wu, Hui[1,2,5]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Dalian Univ Technol, Sch Bioengn, MOE Key Lab Biointelligent Mfg, Dalian, Peoples R China;[3]Shanghai GTLB Biotech Co Ltd, 1688 North Guoguan Rd, Shanghai 200438, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Synthet Biol, Shanghai 200032, Peoples R China;[5]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2025

卷号：416

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20244517339608);WOS:【SCI-EXPANDED(收录号:WOS:001354686700001)】；

基金：This study was supported by the National Key R&D Program of China (2021YFC2103500) , Science and Technology Commission of Shanghai Municipality (21DZ1209100) . Partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering. We want to express our gratitude for the drawing materials provided by BioRender.

语种：英文

外文关键词：Carbon-negative; Syngas-derived acetate; Short-chain carboxylic acids; Acetylation

摘要：Syngas can be efficiently converted to acetate by Moorella thermoacetica under anaerobic conditions, which is environmentally friendly. Coupled with acetate production from syngas, using acetate to synthesize value-added compounds such as short-chain carboxylic acids (SCCAs) becomes a negative-carbon process. Escherichia coli is engineered to utilize acetate as the sole carbon source to produce SCCAs. By knocking out some acetyltransferase genes, introducing exogenous pathway and additional cofactor engineering, the strains can synthesize 3.79 g/L of 3-hydroxypropionic acid (3-HP), 1.83 g/L of (R)-3-hydroxybutyric acid (R-3HB), and 2.31 g/L of butyrate. We used M. thermoacetica to produce acetate from syngas. Subsequently, all engineered E. coli strains were able to produce SCCAs from syngas-derived acetate. The titers of 3-HP, R-3HB, and butyrate are 3.75, 1.68, and 2.04 g/ L, with carbon sequestration rates of 51.1, 26.3, and 38.1 %. This coupled bioprocess has great potential for producing a range of other value-added chemicals from syngas.