文献类型：期刊文献

英文题名：A Highly Sensitive and Selective Near-Infrared Fluorescent Probe for Imaging Peroxynitrite in Living Cells and Drug-Induced Liver Injury Mice

作者：Chai, Xianzhi[1];Li, Bohan[2];Chen, Chi[1];Zhang, Weijian[1];Sun, Lulu[3];Han, Hai-Hao[4,5];Zhang, Yafei[1];Sun, Shasha[1];Yang, Jianming[1];Zhang, Junji[6];He, Xiao-Peng[6,7]

机构：[1]Jiangsu Univ Sci & Technol, Sch Environm & Chem Engn, Zhenjiang 212100, Jiangsu, Peoples R China;[2]Binzhou Med Univ, Sch Integrated Tradit Chinese & Western Med, Featured Lab Biosynth & Target Discovery Act Compo, Yantai 264003, Shandong, Peoples R China;[3]Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Mat Med, Mol Imaging Ctr, Shanghai 201203, Peoples R China;[5]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[6]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,key Lab Adv Mat & Joint Int Re, Shanghai 200237, Peoples R China;[7]Eastern Hepatobiliary Surg Hosp, Natl Ctr Liver Canc, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China

年份：2023

卷号：95

期号：13

起止页码：5747

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20231413840514);WOS:【SCI-EXPANDED(收录号:WOS:000957720400001)】；

基金：ACKNOWLEDGMENTS The authors are grateful to the National Natural Science Foundation of Jiangsu Province (No. BK20210894 and BK20220644) and the National Natural Science Foundation of China (Nos. 22107029, 22122803, 21788102, 21807050, 91853201, 82130099 and 9185920077) .

语种：英文

外文关键词：Diagnosis - Diseases - Fluorescence spectroscopy - Infrared devices - Mammals - Probes

摘要：Drug-induced liver injury (DILI) is a major clinical issue associated with the majority of commercial drugs. During DILI, the peroxynitrite (ONOO-) level is upregulated in the liver. However, traditional methods are unable to timely monitor the dynamic changes of the ONOO- level during DILI in vivo. Therefore, ONOO--activated near-infrared (NIR) fluorescent probes with high sensitivity and selectivity are key to the early diagnosis of DILI in situ. Herein, we report a novel ONOO-- responsive NIR fluorescent probe, QCy7-DP, which incorporates a donor-dual-acceptor pi-electron cyanine skeleton with diphenyl phosphinate. The ONOO--mediated highly selective hydrolytic cleavage via an addition-elimination pathway of diphenyl phosphinate produced the deprotonated form of QCy7 in physiological conditions with a distinctive extended conjugated pi- electron system and similar to 200-fold enhancement in NIR fluorescence emission at 710 nm. Moreover, the probe QCy7-DP was successfully used for the imaging of the endogenous and exogenous ONOO- concentration changes in living cells. Importantly, in vivo fluorescence imaging tests demonstrated that the probe can effectively detect the endogenous generation of ONOO- in an acetaminophen (APAP)-induced liver injury mouse model. This study provides insight into the design of highly selective NIR fluorescent probes suitable for spatiotemporal monitoring of ONOO- under different pathological conditions.