文献类型：期刊文献

中文题名：不同相对分子质量的铁皮石斛多糖对细胞免疫活性的影响

英文题名：Cellular Immune Activity of Dendrobium officinale Polysaccharide with Different Molecular Weight

作者：赵小丹[1];刘玉[1];陶新玉[1];张敏[2];章弘扬[1];王月荣[1];胡坪[1]

机构：[1]华东理工大学化学与分子工程学院、上海市功能性材料化学重点实验室,上海200237;[2]华东理工大学药学院上海市新药设计重点实验室,上海200237

年份：2023

卷号：58

期号：11

起止页码：997

中文期刊名：中国药学杂志

外文期刊名：Chinese Pharmaceutical Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；PubMed;

基金：国家自然科学基金项目资助(81973285)。

语种：中文

中文关键词：铁皮石斛;多糖;相对分子质量;RAW264.7细胞;免疫活性

外文关键词：Dendrobium officinale;polysaccharide;molecular weight;RAW264.7 cells;immune activity

摘要：目的为探究铁皮石斛多糖的免疫调节活性与其相对分子质量之间的关系,通过超声波联合过氧化氢对铁皮石斛多糖进行降解,获得4个不同相对分子质量的铁皮石斛多糖(Dendrobium officinale polysaccharide,DOP)DOP、DOP-30、DOP-60和DOP-150。方法采用高效液相色谱、红外光谱、核磁共振波谱等方法对各铁皮石斛多糖样品的结构进行表征,采用小鼠巨噬细胞RAW264.7构建体外免疫活性实验模型,测定细胞的增殖率、吞噬率、一氧化氮(NO)释放量及细胞因子[肿瘤坏死因子-α(tumor necrosis factor-α)],TNF-α)分泌量,探究铁皮石斛多糖相对分子质量对其免疫调节活性的影响。结果DOP降解后相对分子质量明显降低,降解产物的单糖组成、官能团及糖链主要结构均未发生明显变化,且仍具有三螺旋构象。在12.5~100μg·mL^(-1)范围内,DOP、DOP-30、DOP-60和DOP-150均可提高RAW264.7细胞的NO释放量和TNF-α分泌量,具有良好的免疫调节活性。结论铁皮石斛多糖的免疫调节活性受相对分子质量影响较大,相对分子质量最小的DOP-150的免疫增强能力最低,表明在发挥免疫调节活性时需要一定的链长。
OBJECTIVE In order to explore the relationship between the immunomodulatory activity of Dendrobium officinale polysaccharide(DOP)and its molecular weight.METHODS DOP was degraded by ultrasonic combined with hydrogen peroxide,and four dendrobium officinale polysaccharides with different molecular weight were obtained,including DOP,DOP-30,DOP-60,and DOP-150.The structures of DOP samples were characterized by high performance liquid chromatography,infrared spectroscopy and nuclear magnetic resonance spectroscopy.Mouse macrophage line RAW 264.7 cells were used to construct an experimental model of immune activity in vitro.The proliferation rate,phagocytosis rate,NO release and tumor necrosis factor-α(TNF-α)secretion of the cells were determined to investigate the effect of DOP′s molecular weight on its immunomodulatory activity.RESULTS The molecular weight of DOP decreased significantly after degradation,and the composition of monosaccharide,functional group and main structures of sugar chain of DOP degradation products did not change significantly,and still had the triple helical conformation.In the range of 12.5-100μg·mL^(-1),DOP,DOP-30,DOP-60 and DOP-150 could increase the proliferation,phagocytosis,NO release and TNF-αsecretion of RAW 264.7 cells and showed good immunomodulatory activity.CONCLUSION The immunomodulatory activity of polysaccharides from Dendrobium officinale is significantly affected by the molecular weight,and DOP-150 with the smallest molecular weight has the lowest immunomodulatory activity,which indicating that dendrobium officinale polysaccharides require a certain chain length to exert their immunomodulatory activity.