文献类型：期刊文献

中文题名：基于MIL-100(Fe)的磁性纳米碳材料的制备及其在内源性肽富集中的应用

英文题名：Preparation of magnetic nanoparticles based on MIL-100(Fe) and its application in the enrichment of endogenous peptides

作者：赵雅梦[1];王敏[1];张凌怡[1];鲁誉浩[1];张维冰[1]

机构：[1]华东理工大学化学与分子工程学院

年份：2019

卷号：37

期号：8

起止页码：831

中文期刊名：色谱

外文期刊名：Chinese Journal of Chromatography

收录：CSTPCD;;Scopus;WOS:【ESCI(收录号:WOS:000502578600007)】；北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；PubMed;

基金：National Natural Science Foundation of China (Nos. 21475044, 51472121).

语种：中文

中文关键词：基质辅助激光解吸电离飞行时间质谱;富集;金属有机框架;内源性肽段

外文关键词：matrix-assisted laser desorption ionization-time of flight mass spectrometry ( MALDI -TOF MS);enrichment;metal-organic framework (MOFs);endogenous peptides

摘要：以Fe3O4为核,采用层层自组装的方法合成核壳型Fe3O4@MIL-100(Fe)磁性纳米材料,经高温煅烧得到具有较大孔径(17.78 nm)、高含碳量(6.79%)的磁性纳米材料Fe3O4@MC。将该材料用于多肽的富集,并通过基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF MS)进行鉴定。Fe3O4@MC材料从牛血清白蛋白(BSA)酶解液中富集得到33条肽段,且具有较高的选择性(BSA酶解液与BSA的质量比为1∶400),用于人血清中内源性肽段的分离富集也取得了较好的效果。
Core-shell magnetic nanoparticles,Fe 3O 4@MIL-100(Fe),were synthesized by layer -by-layer self-assembly.The final material,Fe 3O 4@MC,was obtained after high temperature calcination.Fe 3O 4@MC magnetic nanoparticles exhibited large pore size (17.78 nm) and high carbon content (6.79%).Thirty-three peptides of bovine serum albumin (BSA) digest were enriched by Fe 3O 4@MC magnetic nanomaterial.Fe 3O 4@MC magnetic nanomaterial also showed high selectivity for peptides when the mass ratio of BSA digest to BSA was 1∶400.In addition,the nanomaterial material exhibited excellent enrichment performance for the endogenous peptides of human serum.