文献类型：期刊文献

英文题名：Tyrosinase-doped bioink for 3D bioprinting of living skin constructs

作者：Shi, Y.[1];Xing, T. L.[1];Zhang, H. B.[1];Yin, R. X.[1];Yang, S. M.[1];Wei, J.[2];Zhang, W. J.[3]

机构：[1]East China Univ Sci & Technol, Sch Mech & Power Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Mat, Shanghai 200237, Peoples R China;[3]Univ Saskatchewan, Div Biomed Engn, Saskatoon, SK, Canada

年份：2018

卷号：13

期号：3

外文期刊名：BIOMEDICAL MATERIALS

收录：;EI(收录号：20181705057763);WOS:【SCI-EXPANDED(收录号:WOS:000426958200003)】；

基金：The authors would like to thank the financial support to this research received from the following funding agencies: National Natural Science Foundation of China: 21676083, Shanghai Lianmeng Gant (LM201603), and the opening project of the Shanghai Key Laboratory of Orthopedic Implants (KFKT2016001).

语种：英文

外文关键词：bioink; 3D bioprinting; living skin constructs; Tyrosinase

摘要：Three-dimensional bioprinting is an emerging technology for fabricating living 3D constructs, and it has shown great promise in tissue engineering. Bioinks are scaffold materials mixed with cells used by 3D bioprinting to form a required cell-laden structure. In this paper, a novel bioink made of gelatin methacrylamide (GelMA) and collagen (Col) doped with tyrosinase (Ty) is presented for the 3D bioprinting of living skin tissues. Ty has the dual function of being an essential bioactive compound in the skin regeneration process and also as an enzyme to facilitate the crosslink of Col and GelMA. Further, enzyme crosslinking together with photocrosslinking can enhance the mechanical strength of the bioink. The experimental results show that the bioink is able to form stable 3D living constructs using the 3D bioprinting process. The cell culture shows that three major cell lines: human melanocytes (HEM), human keratinocytes (HaCat) and human dermal fibroblasts (HDF) exhibit high cell viabilities. The viability of these three cell lines is above 90%. The proliferation and scratching test show that Ty can enhance the proliferation of HEM, inhibit the growth and migration of HDF and not affect HaCat significantly. Animal tests show that the doped bioinks for 3D bioprinting can help form an epidermis and dermis, and thus have high potential as a skin bioink.