文献类型：期刊文献

英文题名：Roundup? confers cytotoxicity through DNA damage and Mitochondria-Associated apoptosis induction

作者：Hao, Youwu[1];Chen, Hui[1];Xu, Wenping[1];Gao, Jufang[2];Yang, Yun[1];Zhang, Yang[1];Tao, Liming[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China

年份：2019

卷号：252

起止页码：917

外文期刊名：ENVIRONMENTAL POLLUTION

收录：;EI(收录号：20192607098729);WOS:【SCI-EXPANDED(收录号:WOS:000483005500097)】；

基金：This work was financial supported by Shanghai Sailing Program (No.19YF1411100) and Innovation Program of Shanghai Municipal Education Commission (NO.201701070002E00037), Innovation Program of Shanghai Municipal Education Commission, China (NO. 201701070002E00037).

语种：英文

外文关键词：Glyphosate-based herbicide; Cytotoxicity; DNA damage; Mitochondria-associated apoptosis; Human health risk

摘要：Glyphosate-based herbicides (GBH) are the most widely used pesticides in the world. The extensive use of them increases the potential human health risk, including the human inhalation toxicity risk. We studied the effect of the most famous GBH Roundup (R) (RDP) in the concentration range from 50 to 125 mu g/mL on Mitochondria-Associated apoptosis and DNA damage in Human alveolar carcinoma cells (A549 cells). Alkaline comet assay, immunofluorescence assay and Flow Cytometric Analysis assay were employed to detect DNA damages and apoptosis of A549 cells. We found RDP caused concentration-dependent increases in DNA damages and proportion of apoptotic cells in A549 cells. RDP induced the DNA single-strand breaks and double-strand breaks; the collapse of mitochondrial membrane by increasing Bax/Bcl-2, resulting in the release of cytochrome c into cytosol and then activated caspase-9/-3, cleaved poly (ADP-ribose) polymerase (PARP) in human lung tissue cells. The results demonstrate that RDP can induce A549 cells cytotoxic effects in vitro at the concentration lower than the occupational exposures level of workers, which means RDP has a potential threat to human health. (C) 2019 Elsevier Ltd. All rights reserved.