文献类型：期刊文献

英文题名：Soft tissue adhesive composed of modified gelatin and polysaccharides

作者：Mo, XM; Iwata, H; Matsuda, S; Ikada, Y

机构：[1]Kyoto Univ, Inst Frontier Med Sci, Sakyo Ku, Kyoto, Japan;[2]E China Univ Sci & Technol, Inst Mat Sci, Shanghai 200237, Peoples R China

年份：2000

卷号：11

期号：4

起止页码：341

外文期刊名：JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000087993600002)】；

语种：英文

外文关键词：hemostatic agent; gelatin; polysaccharides; gel; dextran; hydroxyethyl starch; Schiff base; adhesive glue

摘要：Although fibrin glue has been clinically used as a surgical adhesive, hemostatic agent, and sealant, it has the risk of virus infection because its components, fibrinogen and thrombin, are obtained from human blood, To circumvent this problem, we employed bioabsorbable gelatin and polysaccharides to prepare a safer hemostatic glue. Gelatin was modified with ethylenediamine using water-soluble carbodiimide to introduce additional amino groups into the original gelatin, while dextran and hydroxyethyl-starch were oxidized by sodium periodate to convert 1,2-hydroxyl groups into dialdehyde groups. Upon mixing of the two polymer components in aqueous solution, Schiff base was formed between the amino groups in the modified gelatin and the aldehyde groups in the modified polysaccharides, which thus resulted in intermolecular cross-linking and gel formation. The fastest gel formation took place within 2 s, and its bonding strength to porcine skin was about 225 gf cm(-2) when 20 wt% of an amino-gelatin (55% amino) and 10 wt% of aldehyde-HES (>84% dialdehyde) aqueous solutions were mixed. In contrast, the gelation time and bonding strength of fibrin glue was 5 s and 120 gf cm(-2), respectively.