文献类型：期刊文献

中文题名：大孔聚甲基丙烯酸缩水甘油酯固定化酶载体的合成及性能研究

英文题名：The Studies on Synthesis and Properties of Glycidyl Methacrylate-N,N′-methylene-bis (acrylamide) Copolymer Supports of Enzyme Catalyst

作者：乌云高娃[1];卢冠忠[1];郭杨龙[1];王筠松[1];魏东芝[2]

机构：[1]华东理工大学工业催化研究所,上海200237;[2]华东理工大学生物反应器国家重点实验室,上海200237

年份：2002

卷号：60

期号：3

起止页码：504

中文期刊名：化学学报

外文期刊名：Acta Chimica Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：大孔聚甲基丙烯酸缩水甘油酯;固定化酶;载体;合成;反相悬浮聚合;青霉素酰化酶

外文关键词：glycidyl methacrylate; N,N '-methylene-bis(acrylamide); inverse suspension copolymerization; penicillin acylase; immobilization

摘要：选用反应性单体甲基丙烯酸缩水甘油酯 ,以N ,N′ 亚甲基双 (丙烯酰胺 )为交联剂 ,甲酰胺为致孔剂 ,用反相悬浮聚合方法制得一系列珠状共聚物载体 ,通过测定其表面的物化性能、表面的环氧基浓度和固定青霉素酰化酶活性 ,讨论了交联剂用量、配比及合成条件对共聚物表面性能和固定化青霉素酰化酶活性的影响 .
Reactive, macroporous and beaded glycidyl methacrylate-N, N'-methylene-bis (acrylamide) copolymers of widely varying crosslink densities were synthesized with formamide as porogenic agent by inverse suspension copolymerization. The effects of crosslink density on structure properties and apparent activity of immobilized penicillin G acylase were investigated. Specific surface area increased, pore volume, porosity and average pore diameter decreased with concomitant increase in crosslinking monomer. Immobilized enzyme activity of 254.8 U/g was obtained with a polymer with of omega = 60% crosslinking having a mean pore diameter of 4.9 nn and an oxirane group content of 0.005 mmol . m(-2).