文献类型：期刊文献

中文题名：Living hybrid material based on probiotic with photothermal properties inhibits PD-L1 expression after tumouricidal photothermal therapy

作者：Ning Jiang[1];Mingyan Jiang[2];Jianshu Chen[1];Ali Mohsin[1];Yuqing Mu[3];Xiaoping Yi[1];Yingping Zhuang[1];Jiangchao Qian[1];Jiaofang Huang[1,2]

机构：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,China;[2]College of Life Sciences,Jiangxi Normal University,Nanchang,Jiangxi Province,China;[3]Institute for biomedicine and glycomics,Griffith University School of Medicine and Dentistry,Griffith University,Queensland,Australia

年份：2025

卷号：6

期号：1

起止页码：73

中文期刊名：Biomaterials Translational

外文期刊名：生物材料转化电子杂志(英文)

基金：supported by the National Key Research and Development Program of China(No.2020YFA0908900);the Natural Science Foundation of Shanghai(No.22ZR1416000);the Open Funding Project of State Key Laboratory of Microbial Metabolism(No.MMLKF24-01).

语种：英文

中文关键词：biomaterials;Escherichia coli Nissle 1917;programmed cell death ligand 1;photothermal therapy

摘要：Photothermal therapy is a safe and effective tumour treatment strategy due to its excellent spatiotemporal controllability.However,interferon gamma in the tumour microenvironment is upregulated after photothermal therapy,which enhances the expression of programmed cell death ligand 1(PD-L1)in tumour cells.This further promotes immunosuppression and tumour metastasis,resulting in a poor prognosis in cancer therapy.Traditional nanodrugs often face challenges in penetrating the dense extracellular matrix of solid tumours,whereas certain probiotics possess the ability to specifically colonise the core regions of tumours.In this research,we used Escherichia coli Nissle 1917(ECN)as a chassis cell and self-assembly polydopamine(PDA)on the ECN surface.The black PDA@ECN(notes as PE)actively colonises at the tumour site and produces a photothermal effect under 808 nm laser irradiation to kill tumour cells.To overcome the high expression of PD-L1 induced after photothermal therapy,metformin(MET)was also encapsulated in PE to form PDA@MET@ECN(notes as PME).In vivo experiments demonstrated that PME effectively inhibited the PD-L1 expression and growth of CT26 tumour cells.Overall,PME reverses the immunosuppressive tumour microenvironment and enhances the effect of photothermal/immune therapy in tumour treatment.