文献类型：期刊文献

英文题名：Promoted Proliferation of Hematopoietic Stem Cells Enabled by a Hyaluronic Acid/Carbon Nanotubes Antioxidant Hydrogel

作者：Zhang, Yuanhao[1];Tong, Yudong[1];Pan, Xiuwei[1];Cai, Haibo[1];Gao, Yun[1];Zhang, Weian[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：304

期号：4

外文期刊名：MACROMOLECULAR MATERIALS AND ENGINEERING

收录：;EI(收录号：20190406403624);WOS:【SCI-EXPANDED(收录号:WOS:000464437200006)】；

基金：This work was financially supported by the National Natural Science Foundation of China (grant numbers 21574039 and 21875063), the Science and Technology Commission of Shanghai Municipality (grant number 15JC1401402), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：antioxidant hydrogels; carbon nanotubes; hematopoietic stem cells; reactive oxygen species

摘要：Reactive oxygen species (ROS) level is closely associated with the physiological function of hematopoietic stem cells (HSCs) in ex vivo culture systems. Previously developed hydrogels in use of HSCs culture have generally failed in considering their antioxidant property. In the present work, antioxidant hydrogels are constructed to evaluate the biological effect of ROS on HSCs proliferation by suppressing oxidative stress damage. Functionalized carbon nanotubes (CNTs), which act as potential antioxidants, are physically encapsulated in biocompatible hyaluronic acid (HA) hydrogels to achieve long-term antioxidant activity. Consequently, these hybrid hydrogels exhibit enhanced physical properties and superior scavenging capability on oxides and peroxides compared to the pure HA hydrogel. The results indicate that ROS significantly inhibit the biological characteristics of HSCs. Nevertheless, the proliferation ability and pluripotency are dramatically improved when the culture system is supplied with the antioxidant hydrogel, revealing that the CNT-incorporated hydrogel can effectively relieve oxidative stress response in HSCs and reduce apoptosis from ROS. Therefore, the HA/CNT hydrogel can provide a novel strategy to establish an artificial microenvironment with a low ROS level for HSCs proliferation.