文献类型：期刊文献

英文题名：Transformation of Geniposide into Genipin by Immobilized β-Glucosidase in a Two-Phase Aqueous-Organic System

作者：Yang, Yi-Shun[1,2];Zhang, Tong[1];Yu, Shai-Cheng[2];Ding, Yue[1];Zhang, Li-Ying[1];Qiu, Chen[2];Jin, Duo[2]

机构：[1]Shanghai Univ Tradit Chinese Med, Expt Ctr Teaching & Learning, Shanghai 201203, Peoples R China;[2]E China Univ Sci & Technol, Coll Chem Engn, Shanghai 200237, Peoples R China

年份：2011

卷号：16

期号：5

起止页码：4295

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000290955800059)】；

基金：Support from National Natural Science Foundation of China (Project Number: 30500667), the National Scientific and Technological Major Special Project of China (Project Number: 2009ZX09103-449), and Shanghai Municipal Education Commission Project (Project Number: J51301, 10SG40) is gratefully acknowledged.

语种：英文

外文关键词：immobilized beta-glucosidase; geniposide; genipin; aqueous-organic two-phase system

摘要：Genipin is the bioactive compound of geniposide and a natural cross-linking agent. In order to improve the preparation process of genipin, the hydrolysis of geniposide to genipin by immobilized beta-glucosidase in an aqueous-organic two-phase system was studied. beta-Glucosidase was immobilized by the crosslinking-embedding method using sodium alginate as the carrier. The optimum reaction temperature, pH value and time were 55 degrees C, 4.5 and 2.5 h, respectively. To reduce genipin hydrolysis and byproduct production the reaction was carried out in an aqueous-organic two-phase system comprising ethyl acetate and sodium acetate buffer. The product was analyzed by HPLC, UV, IR, and NMR. The yield of genipin was 47.81% and its purity was over 98% (HPLC).