文献类型：期刊文献

中文题名：pH和溶解氧浓度对重组酵母表达乙型肝炎表面抗原的影响

英文题名：EFFECT OF pH VALUE AND DISSOLVED OXYGEN CONCENTRATION FOR EXPRESSION OF HEPATITIS B SURFACE ANTIGEN BY GENETICALLY ENGINEERED YEAST IN FERMENTATION PROCESSES

作者：施源[1];沈绿萍[2];唐孝宣[1];李载平[2];袁渭康[1];陈敏恒[1]

机构：[1]华东化工学院;[2]中国科学院上海生物化学研究所;

年份：1989

卷号：5

期号：4

起止页码：284

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：重组酵母;HBsAg;重组质粒;稳定性

外文关键词：Fermentation by genetically engineered yeast; hepatitis B surface antigen;stability of recombinant plasmid

摘要：观察酵母基因工程菌生产目的基因表达产物——乙型肝炎表面抗原的发酵,细胞生长和乙型肝炎表面抗原表达是两个相互影响的过程,因此,存在着pH的调节序列。溶解氧浓度的控制对重组质粒稳定性以及目的基因的高表达具有较大影响,实验获得控制溶解氧浓度为70%饱和度的条件,可使雨组质粒保持73%以上的稳定性,并且。乙型肝炎表面抗原达到98.6的相对浓度。
Cell growth and expression of hepatitis B surface antigen (HBsAg) interact on each other in fermentation to produce exogenous protein HBsAg by genetically engineered yeast S. cerevisiae containing the HBsAg gene. Then there are adjustable sequences of pH values together with the metabolic phenomenon of the two processes. It is important that dissolved oxygen concentration in fermentation should be controlled to maintain the stability of recombinant plasmid and obtain the high expression of HBsAg. Under the condition to control the dissolved oxygen concentration to 70% saturation. The stability of the recombinant plasmid should be maintained at 73% and expressed amount of HBsAg gene achieved 98.6 in relative concentration.