文献类型：期刊文献

英文题名：Electrochemical detection of short DNA sequences related to the escherichia coli pathogen using a zirconia-modified screen-printed DNA biosensor

作者：Zuo, Shao-Hua[1]; Zhang, Ling-Fan[1]; Zhao, Yan-Hui[1]; Yuan, Hui-Hui[2]; Lan, Min-Bo[1,2]; Lawrance, Geoffrey A.[3]; Wei, Gang[4]

机构：[1] Research Centre of Analysis and Test, Institute of Advanced Materials, East China University of Science and Technology, Shanghai 200237, China; [2] Key Laboratory for Ultrafine Materials, Chinese Education Ministry, East China University of Science and Technology, Shanghai 200237, China; [3] Discipline of Chemistry, School of Environment and Life Science, University of Newcastle, Callaghan, NSW 230, Australia; [4] CSIRO Materials Science and Engineering, PO Box 218, Lindfield, NSW 2070, Australia

年份：2008

卷号：61

期号：12

起止页码：962

外文期刊名：Australian Journal of Chemistry

收录：EI(收录号：20085211808097)

语种：英文

外文关键词：DNA sequences - Electrochemical electrodes - Biosensors - Probes - Cyclic voltammetry - Aromatic compounds - Chemical detection - Electrochemical biosensors - DNA - Escherichia coli

摘要：A simple, disposable and inexpensive electrochemical DNA biosensor based on a zirconia (ZrO2) modified thin film screen-printed electrode (ZrO2/SPE) has been developed. Short DNA sequences (21 monomer units) from the Escherichia coli pathogen, modified with a phosphate group at the 5 end, were attached to the surface of the electrode through the affinity of the phosphate group for zirconia, to produce an effective DNA probe (ssDNA/ZrO2/SPE). DNA immobilization and hybridization were characterized using differential pulse voltammetry by employing methylene blue as redox indicator. Target sequences hybridized with the probe resulted in a decrease of the reduction peak current of methylene blue intercalated into the probe. The response of a non-complementary sequence and a single base pair mismatch sequence were both clearly distinguished from that of a complementary sequence. The developed biosensor had a high selectivity and sensitivity towards hybridization detection (10-10 M complementary DNA detectable). Making use of screen-printed technology, the fabrication of the biosensors exhibited satisfactory reproducibility, investigated by cyclic voltammetry and differential pulse voltammetry. The relative standard deviation was found to be 2/SPE) from a batch. ? CSIRO 2008.