文献类型：期刊文献

中文题名：3种抽提隐孢子虫卵囊DNA方法的比较

英文题名：Comparision of Three DNA Template Preparations of Cryptosporidium oocysts

作者：闫东丽[1];李晓虹[2];叶邦策[1]

机构：[1]华东理工大学生物工程学院,上海200237;[2]上海出入境检验检疫局,上海200135

年份：2009

卷号：28

期号：2

起止页码：276

中文期刊名：食品与生物技术学报

外文期刊名：Journal of Food Science and Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：上海市科委技术标准专项(05dz05008)

语种：中文

中文关键词：隐孢子虫;DNA抽提;巢式PCR

外文关键词：Cryptosporidium, DNA extraction, nested-PCR

摘要：采用FTA filter paper、Fast DNA SPIN for Soil Kit、UNIQ-10柱式基因组抽提试剂盒3种方法制备样品DNA模板,根据隐孢子虫SSU rRNA设计内外两对引物进行巢式PCR扩增。3种方法检测的灵敏度分别为1,10,100个/mL。结果表明:FTA filter paper方法简便、特异性强、灵敏度高。
In order to find a more simple, sensitive and specific methods of DNA template preparations from Cryptosporidium oocysts, three methods. FTA filter paper,Fast DNA SPIN for Soil Kit, UNIQ-10 kit was compared. All the primers in the Nested PCR protocol were desighed according to the small-subunit rRNA genes. The sensitivities of three different methods were 1,10,100 oocysts /mL. FTA filter paper has proven to be the more simple, sensitive and specific methods.