文献类型：期刊文献

英文题名：Schultriene and nigtetraene: two sesterterpenes characterized from pathogenetic fungi via genome mining approach

作者：Jiang, Lan[1];Yang, Huanting[1];Zhang, Xue[1];Li, Xiaoying[1];Lv, Kangjie[1];Zhang, Weiyan[1];Zhu, Guoliang[1];Liu, Chengwei[2];Wang, Yongheng[3];Hsiang, Tom[4];Zhang, Lixin[1];Liu, Xueting[1]

机构：[1]East China Univ Sci Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Northeast Forestry Univ, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Coll Life Sci, Harbin, Peoples R China;[3]Jinan Univ, Inst Tradit Chinese Med & Nat Prod, Coll Pharm, Guangdong Prov Key Lab Pharmacodynam Constituents, Guangzhou, Peoples R China;[4]Univ Guelph, Sch Environm Sci, 50 Stone Rd East, Guelph, ON, Canada

年份：2022

卷号：106

期号：18

起止页码：6047

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20223612692075);WOS:【SCI-EXPANDED(收录号:WOS:000847647600002)】；

基金：The study was financially supported by the National Key Research and Development Program of China (2020YFA0907800 and 2019YFA0906200), the National Natural Science Foundation of China (21907031, 81903529, 21977029, 31720103901, 21877124), and the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, the 111 Project (B18022).

语种：英文

外文关键词：Bifunctional terpene synthases; Sesterterpene; Genome mining; Heterologous expression; Cyclization mechanisms

摘要：Fungal bifunctional terpene synthases (BFTSs) have been reported to contribute to the biosynthesis of a variety of di/sesterterpenes via different carbocation transportation pathways. Genome mining of new BFTSs from unique fungal resources will, theoretically, allow for the identification of new terpenes. In this study, we surveyed the distribution of BFTSs in our in-house collection of 430 pathogenetic fungi and preferred two BFTSs (CsSS and NnNS), long distance from previously characterized BFTSs and located in relatively independent branches, based on the established phylogenetic tree. The heterologous expression of the two BFTSs in Aspergillus oryzae and Saccharomyces cerevisiae led to the identification of two new sesterterpenes separately, 5/12/5 tricyclic type-A sesterterpene (schultriene, 1) for CsSS and 5/11 bicyclic type-B sesterterpene (nigtetraene, 2) for NnNS. In addition, to the best of our knowledge, 2 is the first 5/11 bicyclic type-B characterized sesterterpene to date. On the basis of this, the plausible cyclization mechanisms of 1 and 2 were proposed based on density functional theory calculations. These new enzymes and their corresponding terpenes suggest that the chemical spaces produced by BFTSs remain large and also provide important evidences for further protein engineering for new terpenes and for understanding of cyclization mechanism catalyzed by BFTSs.