文献类型：期刊文献

英文题名：Tolerance response and metabolism of acetic acid by biodetoxification fungus Amorphotheca resinae ZN1

作者：Gao, Xiaochuang[1];Gao, Qiuqiang[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：275

起止页码：31

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20181605014611);WOS:【SCI-EXPANDED(收录号:WOS:000432620800006)】；

基金：This research was supported by the National High-Tech Program of China (2012AA022301) and the Natural Science Foundation of China (31300070).

语种：英文

外文关键词：Amorphotheca resinae ZN1; Acetic acid; Detoxification; Pathway; Transcriptome; Tolerance response

摘要：Removal of acetic acid from pretreated lignocellulose biomass is an important step for the consequent fermentation on production of cellulosic ethanol and biobased chemicals. This study elucidates the biological metabolism and tolerance response of acetic acid by a widely used biodetoxification fungus Amorphotheca resinae ZN1. Acetic acid is consumed as a prior substrate to glucose and xylose by A. resinae ZN1, and the consumption is highly accelerated by solid state culture. Acetic acid is metabolized through the tricarboxylic acid (TCA) cycle when glucose exists in the medium, while through the two cycles of both the TCA cycle and glyoxylate cycle when there is no sugar in the medium. The tolerance response of A. resinae ZN1 to acetic acid includes various biological processes such as activation of ions transport, increase in amino acids uptake and biosynthesis, as well as induction of ergosterol biosynthesis and ATP generation. The study provided important basis for the future biodetoxification strain modification for enhanced acetic acid removal.