文献类型：期刊文献

英文题名：Biomimetic Hydroxyapatite Nanorods Promote Bone Regeneration via Accelerating Osteogenesis of BMSCs through T Cell-Derived IL-22

作者：Yu, Fei[1];Lian, Ruixian[2];Liu, Lu[1];Liu, Ting[3];Bi, Chao[4];Hong, Kan[2];Zhang, Shuiquan[2];Ren, Jiazi[5,6];Wang, Haikun[5,6];Ouyang, Ningjuan[1];Du, Lin-Juan[3];Liu, Yuan[3];Zhou, Lujun[3];Liu, Yan[3];Fang, Bing[1];Li, Yulin[2,7];Duan, Sheng-Zhong[3];Xia, Lunguo[1]

机构：[1]Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Orthodont, Shanghai 200125, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat Minist Educ, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Mat Sci & Engn,Minist Educ,Key Lab Ultrafine, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Lab Oral Microbiota & Syst Dis, Shanghai 200125, Peoples R China;[4]Anhui Med Univ, Affiliated Hosp 1, Dept Stomatol, Hefei 230061, Peoples R China;[5]Chinese Acad Sci, Inst Pasteur Shanghai, CAS Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China;[6]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[7]Shanghai Univ, Wenzhou Inst, Wenzhou 325000, Peoples R China

年份：2022

卷号：16

期号：1

起止页码：755

外文期刊名：ACS NANO

收录：;EI(收录号：20220411524974);WOS:【SCI-EXPANDED(收录号:WOS:000743232200001)】；

基金：This work was supported by the National Natural Science Foundation of China (11932012, 32171348, 81991503, 81921002, 81870790, 81772317, and 51973060), the National Natural Science Foundation of China for Innovative Research Groups (51621002), Science and Technology Commission of Shanghai Municipality (19441906200), Shanghai Rising-Star Program (19QA1405200), Innovative Research Team of High-level Local Universities in Shanghai (SSMU-ZDCX20180902, SSMU-ZDCX20180900), and Young Doctor Collaborative Innovation Team of Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine (QC2018-02) for financial support. We would like to thank the Shanghai Institute of Precision Medicine for assistance in flow cytometry, and we are grateful to Shuai Li for her support of data acquisition and analysis.

语种：英文

外文关键词：osteoimmunomodulation; osteogenesis; mandibular bone regeneration; T cell; interluekin-22

摘要：Manipulations of morphological properties of nano-biomaterials have been demonstrated to modulate the outcome of osteoimmunomodulation and eventually osteogenesis through innate immune response. However, the functions and mechanisms of adaptive immune cells in the process of nanobiomaterials-mediated bone regeneration have remained unknown. Herein, we developed bone-mimicking hydroxyapatite (HAp) nanorods with different aspect ratios as model materials to investigate the impacts of the nanoshape features on osteogenesis and to explore the underlying mechanisms focusing on the functions of T cells and T cell-derived cytokines. HAp nanorods with different aspect ratios (HAp-0, HAp-30, and HAp-100) were implanted into mouse mandibular defect models. Micro-CT and hematoxylin and eosin staining demonstrated that HAp-100 had the best osteogenic effects. Flow cytometry analysis revealed that HAp-100 increased the percentage of T cells in injured mandibles. The osteogenic effects of HAp-100 were significantly blunted in injured mandibles of TCR beta(-/-)mice. The Luminex xMAP assay and ELISA showed that HAp-100 induced a marked increase of interleukin (IL)-22 in injured mandibles. In cultured T cells, HAp-100 manifested the best capacity to induce the production of IL-22. Conditioned media from HAp-100-primed T cells promoted osteogenesis and JAK1/STAT3 activation in bone marrow stromal cells, all of which were abolished by neutralizing antibodies against IL-22. In summary, bone-mimicking HAp nanorods with different aspect ratios could regulate osteogenesis through modulation of T cells and IL-22 in the bone regeneration process. These findings provided insights for mediation of the immune response of T cells by nanomaterials on osteogenesis and strategies for designing biomaterials with osteoimmunomodulative functions.