文献类型：期刊文献

英文题名：In Situ Controlled Release of rhBMP-2 in Gelatin-Coated 3D Porous Poly(ε-caprolactone) Scaffolds for Homogeneous Bone Tissue Formation

作者：Zhang, Qingchun[1];Tan, Ke[2];Zhang, Yan[1];Ye, Zhaoyang[2];Tan, Wen-Song[2];Lang, Meidong[1]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：15

期号：1

起止页码：84

外文期刊名：BIOMACROMOLECULES

收录：;EI(收录号：20140417224246);WOS:【SCI-EXPANDED(收录号:WOS:000329879800010)】；

基金：This research was supported by the Fundamental Research Funds for the Central Universities, the National Natural Science Foundation of China (20804015, 31000424), Specialized Research Fund for the Doctoral Program of Higher Education (200802511021, 20100074120009), "Shu Guang" Project of Shanghai Municipal Education Commission, and Program for Changjiang Scholars and Innovative Research Team in University (IRT0825).

语种：英文

外文关键词：Scaffolds (biology) - Cell culture - Bone - Coatings

摘要：In tissue engineering, incorporation of bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable bone formation as well as severe inflammation. In this study, genipin-cross-linked gelatin entrapped with recombinant human BMP-2 (rhBMP-2) was exploited to decorate the interior surface of three-dimensional porous poly(epsilon-caprolactone) (PCL) scaffolds. With gelatin-coating, PCL scaffolds demonstrated enhanced water uptake and improved compressive moduli. Intriguingly, a unique release profile of rhBMP-2 composed of a transient burst release followed by a sustained release was achieved in coated scaffolds. These coated scaffolds well supported growth and osteogenesis of human mesenchymal stem cells (hMSCs) in vitro, indicating the retaining of rhBMP-2 bioactivity. When hMSCs-seeded scaffolds were implanted subcutaneously in nude mice for 4 weeks, better bone formation was observed in gelatin/rhBMP-2-coated scaffolds. Specifically, the spatial distribution of newly formed bone was more uniform in gelatin-coated scaffolds than in uncoated scaffolds, which displayed preferential bone formation at the periphery. These results collectively demonstrated that gelatin-coating of porous PCL scaffolds is a promising approach for delivering rhBMP-2 to stimulate improved bone regeneration.