文献类型：期刊文献

英文题名：Simultaneous Quantification of Hg2+ and MeHg+ in Aqueous Media with a Single Fluorescent Probe by Multiplexing in the Time Domain

作者：Zhang, Ziqian[2];Zhang, Baoyan[2];Qian, Xuhong[1,2];Li, Zhong[2];Xu, Zhiping[2];Yang, Youjun[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2014

卷号：86

期号：23

起止页码：11919

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20145200361248);WOS:【SCI-EXPANDED(收录号:WOS:000345803300065)】；

基金：The work is supported by the Natural Science Foundation and of Shanghai Municipality (no. 11ZR1408800), Shanghai Rising-Star Program (no. 13QA1401200), Innovation Program of Shanghai Municipal Education Commission (no. 12ZZ047), Doctoral Fund of Ministry of Education of China (no. 20110074120008), and the National Natural Science Foundation of China (nos. 21106043, 21372080, and 21236002).

语种：英文

外文关键词：Cost effectiveness - Time domain analysis

摘要：Development of a molecular probe for selective detection of MeHg+ in the presence of Hg2+ is a mission impossible to accomplish. Speciation analysis of two substrates with a single kinetic trace exploiting their differential reactivity toward a single probe, i.e., multiplexing in the time domain, is a cost-effective and powerful alternative. We have developed such a probe (Hg410) for simultaneously quantification of Hg2+ and MeHg+ in aqueous media. Hg410 is designed via the covalent-assembly approach, displays a zero background, and bears a very concise molecular construct. It has harnessed proximity-based catalysis to achieve high reactivity toward Hg2+ and MeHg+. An unprecedentedly low detection limit of ca. 4.6 pM and 160 pM was measured for Hg2+ and MeHg+, respectively.