文献类型：期刊文献

英文题名：Reversible Regulation of Succinate Dehydrogenase by Tools of Photopharmacology

作者：Fu, Wen[1];Shao, Zhongli[1];Sun, Xujuan[1];Zhou, Cong[1];Xu, Zhiping[1];Zhang, Yang[1];Cheng, Jiagao[1];Li, Zhong[1,2];Shao, Xusheng[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab BioreactorEngn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Frontier Sci Res Base Optogenet Tech Cel, Sch Pharm, Shanghai 200237, Peoples R China

年份：2022

卷号：70

期号：14

起止页码：4279

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20221511960478);WOS:【SCI-EXPANDED(收录号:WOS:000790344500008)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 21877039 and 32072441), National Key Research and Development Program of China (2018YFD0200100), and Innovation Program of Shanghai Municipal Education Commission (2017-01-07-00-02-E00037).

语种：英文

外文关键词：regulation; SDH; photochromic ligands; azobenzene; optical control

摘要：Succinate dehydrogenase (SDH) is extremely important in metabolic function and biological processes. Modulationof SDH has been reported to be a promising therapeutic target to SDH mutations. Current measures for the regulation of SDH arescarce, and precise and reversible modulation of SDH still remains challenging. Herein, a powerful tool for reversible optical controlof SDH was proposed and evaluated utilizing the technology of photopharmacology. We reported photochromic ligands (PCLs),azobenzene-pyrazole amides (APAs), that exert light-dependent inhibition effects on SDH. Physicochemical property tests andbiological assays were conducted to demonstrate the feasibility of modulating SDH. In this paper, common agricultural pathogenswere used to develop a procedure by which our PCLs could reversibly and precisely control SDH utilizing green light. This researchwould help us to understand the target-ligand interactions and provide new insights into modulation of SDH.