文献类型：期刊文献

英文题名：Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase

作者：Wang, Dezheng[1];Wang, Cheng[1];Wu, Hui[1];Li, Zhimin[1,2];Ye, Qin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2016

卷号：43

期号：1

起止页码：45

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20242616340381);WOS:【SCI-EXPANDED(收录号:WOS:000367855800006)】；

基金：This study was supported by the National Science Foundation for Young Scientist of China (Grant No. 21406065), the Fundamental Research Funds for the Central Universities (Grant No. 222201313007 & 22A201514042), the National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204) and Shanghai Committee of Science and Technology (Grant No. 13DZ1930202).

语种：英文

外文关键词：Glutathione; Escherichia coli; Bifunctional glutathione synthetase; One-strain system; Energy regeneration

摘要：Glutathione (GSH) is an important bioactive substance applied widely in pharmaceutical and food industries. Due to the strong product inhibition in the GSH biosynthetic pathway, high levels of intracellular content, yield and productivity of GSH are difficult to achieve. Recently, a novel bifunctional GSH synthetase was identified to be less sensitive to GSH. A recombinant Escherichia coli strain expressing gshF encoding the bifunctional glutathione synthetase of Streptococcus thermophilus was constructed for GSH production. In this study, efficient GSH production using this engineered strain was investigated. The cultivation process was optimized by controlling dissolved oxygen (DO), amino acid addition and glucose feeding. 36.8 mM (11.3 g/L) GSH were formed at a productivity of 2.06 mM/h when the amino acid precursors (75 mM each) were added and glucose was supplied as the sole carbon and energy source.