文献类型：期刊文献

中文题名：固定化啤酒酵母细胞合成5’-三磷酸腺苷

英文题名：Biosynthesis of Adenosine 5'-Triphosphate by Using Immobilized Beer Yeast Cells

作者：邱蔚然[1];李玉豆[1];蒋晓明[1];徐茜[1]

机构：[1]华东化工学院生化工程研究所

年份：1989

卷号：15

期号：4

起止页码：451

中文期刊名：华东化工学院学报

收录：国家哲学社会科学学术期刊数据库;Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：三磷酸腺苷;合成;啤酒酵母细胞

外文关键词：carrageenin; yeast; immobilized cells; beer yeast cells; adenosine 5'-triphosphate

摘要：用K-卡拉胶包埋啤酒酵母制成固定化细胞,在0.05mol/L葡萄糖,0.022mol/L腺苷酸,0.012mol/LMgSO_4·7H_2O,0.2mol/L氯化钾,0.2mol/L磷酸钾缓冲溶液(pH7.0)中,35℃反应4小时,5′-三磷酸腺苷转化率可达42%,在50ml三角烧瓶中分批反应,连续使用5次,5′-三磷酸腺苷转化率仍维持在37%以上。
The immobilized beer yeast cells entrapped in K-carrageenan gel beads were used to biosynthesize adenosine 5′-triphosphate from a buffer (pH 7.0) substrate solution containing 0.05 mol/L glucose, 0.022 mol/L AMP, 0.012 mol/L MgSO_4·7_2O, 0.2 mol/L KCl, and 0.2 mol/L potassium phosphate. The biosynthesis reaction temperature is 35℃, the reaction time being 4 hours; the conversion rate of ATP can reach 42%. When the reaction wasrepeated batch wise in a 50ml conical flask, the conversion rate of ATP can remain above 37% after five succesive times.