文献类型：期刊文献

英文题名：DNA damage and cell apoptosis induced by fungicide difenoconazole in mouse mononuclear macrophage RAW264.7

作者：Li, Ruirui[1];Liu, Bin[1,2];Xu, Wenping[1];Yu, Lvnan[1];Zhang, Cheng[3];Cheng, Jiagao[1];Tao, Liming[1];Li, Zhong[1];Zhang, Yang[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Shanghai Qingpu Dist Agr Technol Extens Serv Ctr, Shanghai, Peoples R China;[3]UT Southwestern Med Ctr, Dept Pathol, Dallas, TX USA

年份：2022

卷号：37

期号：3

起止页码：650

外文期刊名：ENVIRONMENTAL TOXICOLOGY

收录：;EI(收录号：20215011303596);WOS:【SCI-EXPANDED(收录号:WOS:000730336700001)】；

基金：Innovation Program of Shanghai Municipal Education Commission, China, Grant/Award Number: 201701070002E00037; Shanghai Sailing Program, Grant/Award Number: 19YF1411100

语种：英文

外文关键词：apoptosis; cytotoxicity; difenoconazole; DNA damage; RAW264; 7 macrophages

摘要：Difenoconazole (DFC) is a typical triazole fungicide. Because of its effective bactericidal activity, it has been widely used in agricultural products such as fruits and vegetables. This study revealed the cytotoxic effect of fungicide DFC on mouse monocyte macrophage RAW264.7. The results showed that the IC50 value of DFC on RAW264.7 cells was 37.08 mu M (24 h). DFC can significantly inhibit the viability of RAW264.7 cells, induce DNA damage and enhance apoptosis. The established cytotoxicity test showed that DFC-induced DNA double strand breaks in RAW264.7 cells. DFC-treated cells showed typical morphological changes of apoptosis, including chromatin condensation and nuclear lysis. In addition, DFC can induce the release of Cyt c, promote the collapse of mitochondrial membrane potential and increase the Bax/Bcl-2 ratio in RAW264.7 cells. Through this research, people further understand the toxicity of DFC and provide a more scientific basis for its safety application and risk management.