文献类型：期刊文献

英文题名：Bioinspired Intrinsic Versatile Hydrogel Fabricated by Amyloidal Toxin Simulant-Based Nanofibrous Assemblies for Accelerated Diabetic Wound Healing

作者：Xuan, Qize[1];Jiang, Feng[2];Dong, Hao[1];Zhang, Wenxue[1];Zhang, Feiyang[2];Ma, Tonghao[1];Zhuang, Jiafeng[1];Yu, Jinlong[2];Wang, Yibing[1];Shen, Hao[2,3];Chen, Chao[1];Wang, Ping[4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn Ctr, Sch Biotechnol, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Dept Orthopaed, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China;[3]Jinjiang Municipal Hosp, Dept Orthopaed, Jinjiang 362200, Peoples R China;[4]Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA

年份：2021

卷号：31

期号：49

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20213710879999);WOS:【SCI-EXPANDED(收录号:WOS:000695622700001)】；

基金：Q.X. and F.J. contributed equally to this work. This work was sponsored by the National Natural Science Foundation of China (Grant Nos. 81772364, 21908059, 41907318, and 21636003), Medical Guidance Scientific Research Support Project of Shanghai Science and Technology Commission (Grant No. 19411962600), the China Postdoctoral Science Foundation (Grant No. 2019M651419), Shanghai Sailing Program (Grant No. 19YF1410900), the Fundamental Research Funds for the Central Universities (Grant No. 22221818014), the Shanghai Post-doctoral Excellence Program (Grant No. 2018011), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering. The authors also thank the Research Centre of Analysis and Test of East China University of Science and Technology for the help with the TEM characterization of hydrogel.

语种：英文

外文关键词：amyloid; chronic wounds; intrinsic versatile hydrogel; peptide assembly; wound dressings

摘要：Persistent microbial infection and decreased neovascularization are common issues associated with diabetic wound treatment. Hydrogel dressings that offer intrinsic antibacterial and angiogenesis-inducing may substantially avoid the use of antibiotics or angiogenic agents. Herein, a versatile hydrogel is fabricated using an amyloid-derived toxin simulant (Fmoc-LFKFFK-NH2, FLN) as building blocks, inspired by the defense strategy of Staphylococcus aureus (S. aureus). The simulant assemblies of the hydrogel function as both matrix components and functional elements for diabetic wound treatment. The hydrogel undergoes quick assembly from random monomers to nanofibrils with abundant b-sheet driven by multiple non-covalent interactions. The developed hydrogel demonstrates excellent biocompatibility and accelerates angiogenesis via hypoxia-inducible factor 1 alpha (HIF-1 alpha) and vascular endothelial growth factor A (VEGFA) signaling as a consequence of its amyloidal structure. The simulant-based nanofibrils endow the hydrogel with broad-spectrum antibacterial activity dominated by a membrane-disruption mechanism. In addition, the hydrogel exhibits excellent performance compared with the commercial hydrogel Prontosan in accelerating wound healing of diabetic mice infected with methicillin-resistant S. aureus (MRSA). This study highlights the fabrication of a single component and versatile hydrogel platform, thereby avoiding the drug-related side effects and complicated preparations and demonstrating its profound potential as a clinical dressing for the management of microbe-infected diabetic wounds.