文献类型：期刊文献

英文题名：Expression and Characterization of a Novel Enantioselective Lipase from Aspergillus fumigatus

作者：Shangguan, Jiao-Jiao[1]; Fan, Li-Qiang[1]; Ju, Xin[1]; Zhu, Qing-Qing[1]; Wang, Fu-Jun[2]; Zhao, Jian[1]; Xu, Jian-He[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; [2] Institute of Chinese Materia Medica, Shanghai University of Chinese Traditional Medicine, Shanghai 201203, China

年份：2012

卷号：168

期号：7

起止页码：1820

外文期刊名：Applied Biochemistry and Biotechnology

收录：EI(收录号：20131016084393)

语种：英文

外文关键词：Aspergillus - Cloning - Fatty acids - Purification - Escherichia coli - Enantioselectivity

摘要：A 1,080-bp cDNA (CGMCC 2873) encoding of a cold-active lipase of Aspergillus fumigatus (AFL67) was cloned and expressed in Escherichia coli for the first time. The new lipase, AFL67, was one-step purified by 8.30 folds through Ni-NTA affinity chromatography with a recovery of 86.8 %. The specific activity of purified AFL67 was 449 U mg-1 on p-NP hexanoate. AFL67 preferentially hydrolyzed p-nitrophenyl esters of short- and medium-chain fatty acids, with p-nitrophenyl hexanoate the maximum. The optimum temperature and pH was 15 C and 7.5, respectively. The purified AFL67 was stable at 10-25 C for 30 min, and in the pH range of 6.0-9.0 for 16 h (at 4 C). Its activity was increased by 47 and 50 %, in the presence of 10 % (v/v) ethanol and isopropanol, respectively. The new lipase AFL67 highly enantioselectively deacylated (S)-α-acetoxyphenylacetic acid (APA) and o-Cl-APA, m-Cl-APA, and p-Cl-APA to (S)-mandelic acid and its derivates. These features render this cold-active novel lipase AFL67 attractive for biotechnological applications in the field of enantioselective synthesis of chiral mandelic acids, o-acylated mandelic acids, and their derivates and detergent additives. ? 2012 Springer Science+Business Media, LLC.