文献类型：期刊文献

中文题名：用于测定营养物质的通用生物传感器

英文题名：An Universal Biosensor for the Determination of Various Kind of Nutrients

作者：储炬[1];李友荣[1];俞俊棠[1]

机构：[1]华东理工大学生化工程系,上海200237

年份：1995

卷号：8

期号：4

起止页码：27

中文期刊名：传感技术学报

外文期刊名：Chinese Journal of Sensors and Actuators

收录：CSTPCD;;Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：生物传感器;醋酸铵;营养物质;测定

外文关键词：biosesordissolving oxygen electrodedetemination of main nourishmentammonium acetate

摘要：报导了一种用于测定培养基中的养分的通用生物传感器，这种生物传感器以溶氧电极为基本传感器，在敏感膜上覆合上一种生物膜，用以测定一种假单孢菌培养液的醋酸铵的含量，其线性范围为：０－４０ｍｇ／Ｌ；９０％的响应时间为：１５－２０ｍｉｎ；测定一个样品所需时间为４０－６０ｍｉｎ；其测量精度为：±６％；使用寿命可达一年以上。
A dissolved oxygen probe covered with a biomembranc can be used for the de-termination of a main substrate, ammonium acetate in a culture broth. The specifica-tions of this proposed biosensor were as follows: range of linearity, 0~40 mg/L; re-sponse time (90% ), l5~20 min; time for sample estimation, 40~60 min; precision.±6% FSD; lifetime of usage, >la. The operation of the proposed biosensor is simple,and the cost for the determination of a sample is low. In general, titled biosensor can beapplied to any other substrates which can be oxidized by the organism immobilized ontothe surface of the DO sensor. Preliminary study of on-line estimation of ammonium ac-etate with proposed biosensor was undertaken as well.